27种中药提取物抑制血管生成活性的评价

对27种中药分别采用石油醚、乙酸乙酯、水进行系统提取，利用转基因斑马鱼对提取物进行血管生成抑制活性检测。提取物处理斑马鱼胚胎24 h后，统计斑马鱼节间血管(ISV)的生长情况，并计算抑制率；部分有活性的提取物进一步采用鸡胚尿囊膜（CAM）实验进行验证。实验结果显示，川芎水提物、白芍乙酸乙酯提取物、竹黄水提物、鬼箭羽乙酸乙酯提取物和水提物、乳香水提取物、大青叶乙酸乙酯提取物显示出抑制血管活性现象；其中川芎水提取物、鬼箭羽乙酸乙酯和水提物、乳香水提取物对鸡胚尿囊膜血管生成具有明显的抑制作用。从27种中药中发现了7种提取物具有抑制转基因斑马鱼节间血管生长的作用，这种作用与血管内建生长因子受体2(VEGFR2)表达有关。

Evaluation of anti angiogenetic activity of the extracts from 27 traditional Chinese herbs

We extracted 27 traditional Chinese herbs with petroleum ether, ethyl acetate and water, and then determined the anti angiogenic activity of the extracts with a transgenic zebrafish model (VEGFR2:GFP). We further calculated the intersegmental vessels（ISV）of the zebrafish embryos and the inhibitory rate after 24 h treatment with different extracts. We eventually tested the activity of some active extracts with chick embryo chorioallantoic membrane (CAM). Results show that anti angiogenic activity is exhibited for water extract of Rhizoma Ligustici Chuanxiong,ethyl acetate extract of Radix Paeoniae alba, water extract of Stroma Shiraiae,water extract and ethyl acetate extract of Euonymus alatus,water extract of Boswellia carterii Birdw. and ethyl acetate extract of Folium Isatidis.Angiogenesis of CAM is significantly inhibited for water extracts of Rhizoma Ligustici Chuanxiong,water extract and ethyl acetate extract of Euonymus alatus and water extract of Boswellia carterii Birdw. We discover that 7 extracts of traditional Chinese herbs can inhibit the sprout and growth of ISV of a transgenic zebrafish, which is relevant to the expression of vascular endothelial grouth factor (VEGFR2).