| HPLC法同时测定忍冬的花、叶中木犀草苷和绿原酸含量 |
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| 引用本文： | HPLC法同时测定忍冬的花、叶中木犀草苷和绿原酸含量.HPLC法同时测定忍冬的花、叶中木犀草苷和绿原酸含量[J].山东科学,2013,26(4):20-23. |
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| 作者姓名： | HPLC法同时测定忍冬的花、叶中木犀草苷和绿原酸含量 |
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| 作者单位： | 1. 山东省分析测试中心，山东 济南 250014；
2. 山东师范大学生命科学学院，山东 济南 250014；
3. 山东亚特生态技术有限公司，山东 临沂 266071 |
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| 基金项目： | 十二五国家科技支撑计划（2011BAI06B01）；山东省科技发展计划（2009GG10009043；20100816632） |
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| 摘 要： | 建立了HPLC法测定忍冬的花、叶中木犀草苷和绿原酸含量的方法。应用Shim-pack ODS色谱柱 (4.6×250 mm, 5 μm)，乙腈/0.2 %甲酸水梯度洗脱，流速为1.0 mL/min，检测波长为350 nm。在此色谱条件下，各组分在30 min内均得到良好分离。用该方法测定了3个品种忍冬的花、叶中木犀草苷和绿原酸的含量，结果表明亚特、意大利和九丰一号忍冬叶中木犀草苷含量分别为5.856、2.454和4.633 mg·g-1，明显高于花。
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| 关 键 词： | 忍冬 花 叶 木犀草苷 绿原酸 HPLC |
| 收稿时间： | 2012-10-03 |
Determination of luteoloside and chlorogenic acid in flowers and leaves from Lonicera japonica Thunb. with HPLC |
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| SHI Xin-Gang,SHU Xi-Kai,LIU Wei,LIU Feng,LI Sheng-Bo,WANG Xiao,WANG Dai-Jie.Determination of luteoloside and chlorogenic acid in flowers and leaves from Lonicera japonica Thunb. with HPLC[J].Shandong Science,2013,26(4):20-23. |
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| Authors: | SHI Xin-Gang SHU Xi-Kai LIU Wei LIU Feng LI Sheng-Bo WANG Xiao WANG Dai-Jie |
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| Institution: | 1. Shandong Analysis and Test Center, Shandong Academy of Sciences, Jinan 250014, China;
2. School of Life Sciences, Shandong Normal University, Jinan 250014, China;
3. Shandong Yate Eco tech Co. Ltd., Linyi 266071, China |
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| Abstract: | We constructed a determination approach for the contents of luteoloside and chlorogenic acid in flowers and leaves from Lonicera japonica Thunb. with HPLC. Their compounds were completely separated under the conditions of Shim pack ODS chromatographic column, gradient elution of Acetionitrile and 0.2% methanoic acid, flow rate of 1.0 mL/min and detection wavelength of 350 nm. Results show that luteoloside content in the leaves of Yate, Italy and Jiufengyihao is 5.856, 2.454 and 4.633 mg·g-1, much higher than that in their flowers. |
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| Keywords: | Lonicera japonica Thunb flower leaf luteoloside chlorogenic acid HPLC |
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