| 龙须菜多糖的提取、分离及抗氧化活性的研究 |
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| 引用本文： | 陈美珍,王欣,余杰.龙须菜多糖的提取、分离及抗氧化活性的研究[J].汕头大学学报(自然科学版),2005,20(2):37-42. |
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| 作者姓名： | 陈美珍 王欣 余杰 |
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| 作者单位： | 汕头大学理学院生物系,广东,汕头,515063 |
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| 摘 要： | 对龙须菜多糖的最适提取工艺条件、分离方法及其抗氧化活性进行了研究. 结果表明: 提取多糖的最适条件为浸提温度90℃, 浸提时间4 h, 料水比1∶100. 分别经DEAE 纤维素离子交换层析柱和Sephadex 100层析柱分离, 均显示出提取的多糖含有3种以上组分. 抗氧化实验显示该多糖对Fenton体系产生的·OH自由基具有较强的清除能力.
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| 关 键 词： | 龙须菜 多糖 提取分离 抗氧化 |
| 文章编号： | 1001-4217(2005)02-0037-06 |
| 修稿时间： | 2004年11月29 |
Studies on Extraction, Purification and Antioxidation Function of Polysaccharide from Gracilaria Lemaneiformis |
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| CHEN Mei-zhen,WANG Xin,YU Jie.Studies on Extraction, Purification and Antioxidation Function of Polysaccharide from Gracilaria Lemaneiformis[J].Journal of Shantou University(Natural Science Edition),2005,20(2):37-42. |
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| Authors: | CHEN Mei-zhen WANG Xin YU Jie |
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| Abstract: | The hot water extraction of the polysaccharide from Gracilaria Lemaneiformis was studied.The effects of three factors such as the temperature of extraction,the time of extraction and the ratio of material-water were investigated.The optimum conditions of extraction were obtained as follows:adding 100 times water as much as lamaneiformis in 90℃ for 4 hours.The polysaccharide was further processed by alcohol-precipitating and protein-depositing with chlor-acetic acid.The results of DEAE column chromatography and Sephadex G-100 column chromatography showed that there were at least 3 kinds of polysaccharides in Lemaneiformis.The result of the antioxidation experiment indicated that the polysaccharide had rather strong scavenging activity on hydroxyl radical(·OH). |
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| Keywords: | Gracilaria Lemaneiformis polysaccharide extraction antioxidation |
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