类人胶原蛋白对BHK-21细胞生长的影响

目的研究类人胶原蛋白HLC（Human—like Collagen）对于细胞生长、细胞凋亡、细胞生长周期及细胞形态的影响。方法用体外细胞培养法，选用BHK-21细胞系为实验对象，进行MTT检测、LDH测定、流式细胞分析、形态学观察及统计学分析。结果MTT检测表明0．2，0．3g／LHLC对于细胞生长有显著的促进作用；HLC对于细胞培养液中LDH浓度无显著性影响；经0．1，0．2，0．3g／LHLC作用后的细胞凋亡百分数变化不大，进一步作周期分析，也无显著影响；形态学观察表明0．3g／LHLC对于细胞形态有加长作用。结论HLC可能通过影响细胞膜、增强细胞贴壁生长而促进细胞生长。

Experimental research of human-like collagen on BHK-21 cell growth

Aim To study the effects of Human-like Collagen(HLC) on cell growth by using BHK-21 cell in vitro.Methods The effects of HLC on cell proliferation,cell apoptosis rate,G1,G2,S of cell growth and morphology were studied by MTT Assay,LDH analysis,FCM and morphology observation.Results The present study showed that RPMI1640 media in the presence of Human-like Collagen concentrations at 0.02%,0.03%,had positive effects on cell growth(p<0.05),but had no significant influences on LDH content in culture media.FCM analysis demonstrated that HLC 0.1,0.2,0.3 g/L made a slight change on cell apoptosis rate,and HLC 0.3 g/L had no significant difference in G1,G2,S compared with blank control.Morphology observation showed that HLC 0.3 g/L elongated cells and made them become slender.Conclusion HLC may cause cell proliferation by affecting cell surface and enhancing cell adhesion.