Inhibition of firefly bioluminescence by scavengers of singlet oxygen,superoxide radicals and hydroxyl radicals |
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Authors: | Ch Richter |
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Institution: | (1) C. N. R. Unit for the Study of Physiology of Mitochondria and Institute of General Pathology, University of Padova, Italy |
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Abstract: | Summary The participation of highly energetic oxygen species in the ATP-induced bioluminescence of a firefly-extract has been investigated. The inhibition of light emission by a variety of specific scavengers suggests that singlet oxygen, superoxide radicals and hydroxyl radicals are important intermediates in the firefly bioluminescence reaction.Acknowledgments. I thank Prof. R. Bachofen, Institut für Allgemeine Botanik, Abteilung Mikrobiologie, Universität Zürich, Switzerland, in whose laboratory most of these studies have been performed, for his cooperativity. Financial support by the Deutsche Studienstiftung is gratefully acknowledged. |
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