细裂辽藁本愈伤组织培养及试管苗分化的研究

为保护野生药用植物资源，以细裂辽藁本的茎段为材料，采用组织培养方法，进行了愈伤组织诱导培养、分化培养，不定芽试管苗培养、试管苗生根继代繁殖培养，以及试管苗的移栽与定植的研究．结果证明：2，4-D2．0mg／L，ZT0．4mggL，6-BA0．8mg／L是愈伤组织诱导培养的适宜生长调节剂组合；NAA0．05mg／L与ZT0．6mg／L是愈伤组织不定芽分化培养的适宜生长调节剂组合；1，2MS＋蔗糖16g／L＋NAAO．1mg／L＋IAA0．3mg／L是试管苗繁殖培养的适宜培养基．试管苗移栽成活率为96.2％；定植成活率为99．1％；定植的试管苗保持了野生细裂辽藁本的植物学性状．

The Callus Culture and Differentiation of Tube Seedlings with Ligusticum jeholense Nakai et Kitag var tenuisectum

For the protection of the wild resources, tissue culture methods were used to study the induction culture and differentiation culture of callus, adventitious bud of test-tube seedling culture, subculture,transplanting and colonization. The results showed that 2, 4-D 2.0 rag/L, ZT 0.4 mg/L and 6-BA 0.8 mg/L was suitable growth regulator group of induction of callus culture; NAA 0.05 mg/L and ZT 0.6 mg/L was appropriate growth regulator group of differentiation culture; 1/2MS＋ sucrosel6 g/L＋NAA 0.1 mg/L＋IAA 0.3 mg/L was the suitable culture medium to propagation of test-tube seedling. The survival rate of test-tube seedling was 96.2%, and the colonization survival rate was 99.1%. The colonization of test-tube seedling maintained all biological characters of wild of cracking of Ligusticum jeholense Nakai et Kitag var tenuisectum.