高效液相色谱法分析聚甘油乳酸酯的组成

建立了HPLC-ELSD法测定聚甘油乳酸酯组成的方法.采用Agilent DZORBAX NH2色谱柱（4．6 mm×250 mm），确定了最佳色谱条件：流动相为乙腈和水（体积比为90∶10），蒸发光散射检测器（ELSD），流速为1．2 mL/min，柱温40℃，进样量4μL .结果表明，在0．1～0．5 mg/mL范围内线性关系良好，相关系数为0．9986，在信噪比（S/N）为3时，检出限为0．020 mg/mL .回收率为98％～102％，相对标准偏差（RSD）为1．2％～1．4％.

Determination of the Composition of Polyglycerol Lactic Acid Ester by HPLC

A method for the determination of polyglycerol lactic acid ester by HPLC-ELSD was established. Agilent DZORBAX NH2 column （4.6 mm×250 mm） was used and the optimal chromatographic conditions were determined. with acetonitrile-water（90∶10） as the mobile phase,Vsing evaporative light scattering detector（ELSD）,the flow r ate 1.2 mL/min,column temperature 40 ℃,and the injection volume 4 μL. The results showed that the linear relationship was good in 0.1~0.5 mg/mL,and the correlation coefficient was 0.998 6. The detection limit was 0.020 mg/mL when signal to noise ratio（S/N） was 3. The recovery was 98%~102% and the relative standard deviation（RSD） was 1.2%~1.4%.