薄荷脱毒苗的离体培养及快速繁殖

探讨薄荷(Mentha hoplocalyx Briq)离体培养过程，为优良品种的快速繁殖奠定基础。以薄荷脱毒苗带茎节为外植体，采用MS基本培养基，附加不同激素进行实验。培养基中附加激素6-BA有利芽的发生；适宜的6-BA浓度为1mg／L，出芽率高且出芽数量多；对愈伤组织的增殖以添加2，4-D0．5mg／L、NAA0．5mg／L和6-BAl．Omg／L为最佳。通过薄荷离体培养的研究，获得了较高的植株再生频率，建立有效的组织培养再生体系。

In Vitro Culture and Rapid Propagation of Non-virs Plant of Menthe

To study the in vitro culture of Mentha haplocalyx(Briq.)so as to lay a foundation for the rapid propagation of its improved breeds. The explants used for culture were stem segments with node from Mentha. The media were Ms basal media containing different plant hormones. The media containing 6-BA were effective to induce shoot formation. Optimal BA concentration for shoot and growth was 1mg/L; The best medium with the addition of 2,4-D(0.5mg/L),NAA(0.5mg/L),and 6-BA(1.0mg/L) gave optimal cell. An effective plant regeneration system has been established for Mentha haplicalyx on the basis of factors affecting the culture in vitro.