Proteomic analysis of long-term salinity stress-responsive proteins in <Emphasis Type="Italic">Thellungiella halophila</Emphasis> leaves |
| |
Authors: | Fei Gao YiJun Zhou LingYun Huang DaCheng He GenFa Zhang |
| |
Institution: | (1) Key Laboratory for Cell Proliferation and Regulation Biology of Ministry of Education, College of Life Science, Beijing Normal University, Beijing, 100875, China |
| |
Abstract: | Salinity is one of the most severe environmental factors that may impair crop productivity. A proteomic study based on two-dimensional
gel electrophoresis is performed in order to analyze the long-term salinity stress response of Thellungiella halophila, an Arabidopsis-related halophyte. Four-week-old seedlings are exposed to long-term salinity treatment. The total crude proteins are extracted
from leaf blades, separated by 2-DE, stained with Coomassie Brilliant Blue, and differentially displayed spots are identified
by MALDI-TOF MS or QTOF MS/MS. Among 900 protein spots reproducibly detected on each gel, 30 spots exhibit significant change
and some of them are identified. The identified proteins include not only some previously characterized stress-responsive
proteins such as TIR-NBS-LRR class disease resistance protein, ferritin-1, and pathogenesis-related protein 5, but also some
proteins related to energy pathway, metabolism, RNA processing and protein degradation, as well as proteins with unknown functions.
The possible functions of these proteins in salinity tolerance of T. halophila are discussed and it is suggested that the long-term salinity tolerance of T. halophila is achieved, at least partly, by enhancing defense system, adjusting energy and metabolic pathway and maintaining RNA structure.
Supported by the National Basic Research and Development Program of China (Grant Nos. 2006CB100100, 2004CB117303), National
Natural Science Foundation of China (Grants Nos. 30670203, 30570434), Beijing Municipal National Natural Science Foundation
(Grant No. 5062021), CUN 985-3-3 and Open Fund of Key Laboratory of Cell Proliferation and Regulation Biology, Ministry of
Education of China. |
| |
Keywords: | Thellungiella halophila proteomics salinity stress mass spectrometry two-dimensional gel electrophoresis |
本文献已被 维普 SpringerLink 等数据库收录! |
| 点击此处可从《中国科学通报(英文版)》浏览原始摘要信息 |
| 点击此处可从《中国科学通报(英文版)》下载免费的PDF全文 |
|