hTR基因反义表达质粒构建及序列分析

从人的脐带血管内皮细胞中提取基因组 DNA,通过 PCR方法扩增得到了人端粒酶RNA成分 ( human telomere RNA,h TR)的基因片段 ,扩增产物经酶切克隆到逆转录病毒载体p LNCX上 ,构建了 h TR基因的反义表达质粒 .序列分析结果表明 ,PCR扩增得到的 h TR基因的 DNA序列与所发表的序列完全一致 .构建的反义表达载体中的目的基因正确地反向插入到逆转录病毒载体 p LNCX的克隆位点上 ,构成了 h TR基因的反义表达质粒

Construction of antisense expression plasmid and sequences of hTR gene

Genomic DNA was extracted from blood vessel endothelial cell of umbilical cord of the human, from which the hTR (human telomerase RNA)gene was amplified by PCR. The PCR products by restriction endonuclease were cloned to retroviral expression vector pLNCX. Sequencing shows that the amplified sequences of hTR gene are completely consistent with the published sequences. The antisense expression plasmid of hTR gene is constructed with the target gene trans inserted into retroviral expression vector pLNCX.