| 基于DNA探针和FISH技术对两种有害浮游植物的检测研究 |
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| 引用本文： | 侯建军,李家园,朱祎,贺云彦,胡俊. 基于DNA探针和FISH技术对两种有害浮游植物的检测研究[J]. 湖北师范学院学报(自然科学版), 2014, 0(1): 20-26 |
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| 作者姓名： | 侯建军 李家园 朱祎 贺云彦 胡俊 |
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| 作者单位： | [1]湖北师范学院污染物分析与资源化技术湖北省重点实验室,湖北黄石435002 [2]湖北师范学院生命科学学院,湖北黄石435002 [3]华东师范大学河口海岸学国家重点实验室,上海200062 |
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| 基金项目： | 国家自然科学基金资助项目（编号：41171045）、淡水生态与生物技术国家重点实验室开放课题（编号：2008FB005）;农业部淡水鱼类种质资源与生物技术重点实验室开放课题（编号：LFB20070611）、教育部博士点基金琐目（编号：20070504076）、黄石市2010年度科技计划项目（编号：2010A1019-7）、湖北师范学院2008年度校级创新团队项目,湖北师范学院研究生创新基金项目（编号：201204）资助. |
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| 摘 要： | 针对两种典型的有害浮游植物微小原甲藻和Takayama pulchellum(T.pulchellum)各设计了4条特异性探针,并采用基于PCR管载体的荧光原位杂交检测方法检测了这些探针的标记效果。实验结果表明,针对微小原甲藻和T.pulchellum核糖体大、小亚基DNA(LSU和SSU rDNA)的序列信息所设计的8条荧光标记探针均能够特异性地识别这些目标藻。各探针的标记效果有一定差异,经过标记的目标藻细胞在单种和自然水样混合样品中均可以通过荧光显微镜进行识别和区分。针对微小原甲藻和T.pulchellum的荧光原位杂交检测方法的建立将有助于对样品中这些目标藻进行快速准确地检测和监测。
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| 关 键 词： | 有害浮游植物 寡核苷酸探针 荧光原位杂交 微小原甲藻(Prorocentrum minimum) Takayama pul-chellum |
Identification of two species of harmful photoplankton using fluorescence in situ hybridization and DNA probes |
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| HOU Jian-jun,LI Jia-yuan,ZHU Yi,HE Yun-yan,HU Jun. Identification of two species of harmful photoplankton using fluorescence in situ hybridization and DNA probes[J]. Journal of Hubei Normal University(Natural Science), 2014, 0(1): 20-26 |
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| Authors: | HOU Jian-jun LI Jia-yuan ZHU Yi HE Yun-yan HU Jun |
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| Affiliation: | 1. Hubei Key Laboratory of Pollutant Analysis ＆ Reuse Technology, Huangshi 435002, China; 2. College of Life Science, Hubei Normal University, Huangshi 435002,China; 3. State Key Laboratory of Estuarine and Coastal Research, East China Normal University, Shanghai 200062, China) |
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| Abstract: | Four specific DNA probes were designed to identify Prorocentrum minimum and Takayama pulchellum two harmful phytoplankton, and fluorescence in situ hybridization probes based on PCR tube carriers were developed .Epifluorescence mi-crographs showed that these specific probes labeled with fluorescein isothiocyanate can identified target sequences , and the flu-orescence signal resulted from whole -cell hybridization presented differences .The hybridization cell can be identified in pured and mixed natural algal samples .The results suggested that method of P.minimum and T.pulchellum fluorescence in situ hybridization based DNA probes could achieve fast and accurately detection of target algaes . |
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| Keywords: | harmful phytoplankton oligonucleotide probes fluorescence in situ hybridization （FISH） prorocentrum mini-mum Takayama pulchellum |
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