| 低温胁迫对拟南芥G蛋白突变体SOD、POD酶活性和丙二醛含量的影响 |
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| 引用本文： | 史冬燕.低温胁迫对拟南芥G蛋白突变体SOD、POD酶活性和丙二醛含量的影响[J].贵州大学学报(自然科学版),2009,26(2):14-16. |
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| 作者姓名： | 史冬燕 |
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| 作者单位： | 云南大学生命科学院,云南,昆明,650091;山东菏泽学院生命科学系,山东,菏泽,274015 |
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| 基金项目： | 山东省教育厅科技计划项目，云南省教育厅科学研究基金项目 |
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| 摘 要： | 采用分光光度法测定了低温胁迫下两种拟南芥G蛋白突变体的超氧化物歧化酶（SOD）、过氧化物酶（POD）的活性及丙二醛（MDA）含量的变化。结果表明：在低温12h，野生型（Ws）和突变体gpal-1的SOD酶活性迅速升高，分别为15．501U／mgprotein和9．453U／mgpro—tein，突变体gpal-2则在低温24h升至最高点，酶活性为6．325U／mgprotein。低温胁迫下，三种材料的POD酶活性都呈现降低趋势，而MDA含量呈上升趋势。初步证明外界低温刺激与植物细胞内G蛋白有一定的关联。
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| 关 键 词： | 拟南芥 低温胁迫 POD SOD MDA |
Effect of Low Temperature Stress on Activity of POD,SOD and Content of MDA in G Protein Mutants of Arabidopsis thanliana |
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| SHI Dong-yan.Effect of Low Temperature Stress on Activity of POD,SOD and Content of MDA in G Protein Mutants of Arabidopsis thanliana[J].Journal of Guizhou University(Natural Science),2009,26(2):14-16. |
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| Authors: | SHI Dong-yan |
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| Institution: | 1.Biotechnology Department of Yunnan University;Kunming;650091;China;2.Biology Department of Heze university;Heze 274015;China |
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| Abstract: | Enzymatic activities of SOD,POD and content of MDA of two G protein mutants of Arabidopsis thanliana were analyzed by means of spectrophotometer under low temperature stress.The results show that at 12 h of low temperature stress,the activities of SOD rose up in wide type and gpa1-1,respectively were 15.501 U/mg protein and 9.453 U/mg protein,but gpa1-2 mutant rose up at 24 h of low temperature,the activity was 6.325 U/mg protein.Under low temperature stress,the activities of POD from three kind ingredients... |
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| Keywords: | POD SOD MDA |
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