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| 与小麦赤霉病抗性紧密连锁的基于AFLP片段的STS标记开发(英文) | |
| 引用本文： | 郭培国,Guihua Bai,李荣华,缪绅裕,Dina El-Khishin,陶文琴,陈建辉,谢国文,郑燕玲.与小麦赤霉病抗性紧密连锁的基于AFLP片段的STS标记开发(英文)[J].广州大学学报(自然科学版),2009,8(1). |
| 作者姓名： | 郭培国 Guihua Bai 李荣华 缪绅裕 Dina El-Khishin 陶文琴 陈建辉 谢国文 郑燕玲 |
| 作者单位： | 1. 广州大学生命科学学院,广东,广州,510006 2. USDA-ARS- Plant Science and Entomology Research Unit, 4008 Throckmorton Hall, Manhallan, KS 66506, USA 3. Agricultural Genetic Engineering Research Institute of Egypt, Giza 12619, Carlo, Egypt |
| 基金项目： | 国家自然科学基金，广东省科技计划，教育部回国人员科研启动资金资助项目，广州市科技计划，广州市教育局科研项目 |
| 摘 要： | DNA序列标签位点(STS)是一个操作简便和花费低的分子标记体系,将与某一性状密切连锁的AFLP(扩增片段长度多态性)片段转换成STS标记可直接用于分子育种工作中.本研究利用Ning 7840和Clark的重组自交系及AFLP技术,探测到一个与小麦赤霉病抗性紧密连锁的坐落在染色体3BS的主效数量特性位点(QTL),发现5个Pstl-AFLP片段与该QTL显著关联;其中2个片段与赤霉病抗性达到50%左右的表型变异解析度,一个为35个碱基的相引相片段,另一个为222个碱基的相斥相片段.222个碱基的DNA片段被克隆和测序,发现11个克隆中含有5种不同的DNA序列,其中一种序列在5个克隆中完全一致,该序列被用来作为设计STS标记的DNA模板.经多次实验,开发出了一个共显性STS标记.该STS标记与原222个碱基的AFLP片段谱带(banding pattern)完全一致,具有鉴别小麦育种材料赤霉病抗病强弱和加速抗病育种进程的潜力. |
| 关 键 词： | 扩增片段长度多态性 转换 序列标签住点 小麦 |
Development of a STS marker from an AFLP tightly linked to the major QTL for scab resistance in wheat |
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| GUO Pei-guo,Guihua Bai,LI Rong-hua,MIAO Shen-yu,Dina El-Khishin,TAO Wen-qin,CHEN Jian-hui,XIE Guo-wen,ZHENG Yan-ling.Development of a STS marker from an AFLP tightly linked to the major QTL for scab resistance in wheat[J].Journal og Guangzhou University:Natural Science Edition,2009,8(1). | |
| Authors: | GUO Pei-guo Guihua Bai LI Rong-hua MIAO Shen-yu Dina El-Khishin TAO Wen-qin CHEN Jian-hui XIE Guo-wen ZHENG Yan-ling |
| Institution: | 1.School of Life Sciences;Guangzhou University;Guangzhou 510006;China;2.USDA-ARS-Plant Science and Entomology Research Unit;4008 Throckmorton Hall;Manhattan;KS 66506;USA;3.Agricultural Genetic Engineering Research Institute of Egypt;Giza 12619;Cario;Egypt |
| Abstract: | Conversion of AFLP(Amplified fragment length Polymorphism) markers into sequence-tagged site(STS) markers may generate breeder-friendly markers for marker-assisted selection(MAS).One major QTL on chromosome 3BS was mapped by using AFLPs and the recombinant inbred population from the cross of Ning 7840/Clark.Fine mapping of the QTL region identified five PstI-AFLP markers significantly associated with the major QTL.Two of them individually explained up to 50% of phenotypic variation for scab resistance.One 3... |
| Keywords: | AFLP conversion STS marker wheat |
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