| Effects of Quenching Temperature and Time on Pore Diameter of Poly（3-hydroxybutyrate-co-3-hydroxyhexanoate） Porous Scaffolds and MC3T3-E1 Osteoblast Response to the Scaffolds |
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| 引用本文： | 奚静 李静 朱琳 公衍道 赵南明 张秀芳. Effects of Quenching Temperature and Time on Pore Diameter of Poly（3-hydroxybutyrate-co-3-hydroxyhexanoate） Porous Scaffolds and MC3T3-E1 Osteoblast Response to the Scaffolds[J]. 清华大学学报, 2007, 12(4): 366-371. DOI: 10.1016/S1007-0214(07)70055-X |
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| 作者姓名： | 奚静 李静 朱琳 公衍道 赵南明 张秀芳 |
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| 作者单位： | State Key Laboratory of Biomembrane and Membrane Biotechnology Department of Biological Sciences and Biotechnology Tsinghua University，State Key Laboratory of Biomembrane and Membrane Biotechnology Department of Biological Sciences and Biotechnology Tsinghua University，State Key Laboratory of Biomembrane and Membrane Biotechnology Department of Biological Sciences and Biotechnology Tsinghua University，State Key Laboratory of Biomembrane and Membrane Biotechnology Department of Biological Sciences and Biotechnology Tsinghua University，State Key Laboratory of Biomembrane and Membrane Biotechnology Department of Biological Sciences and Biotechnology Tsinghua University，State Key Laboratory of Biomembrane and Membrane Biotechnology Department of Biological Sciences and Biotechnology Tsinghua University，Beijing 100084 China，Beijing 100084 China，Beijing 100084 China，Beijing 100084 China，Beijing 100084 China，Beijing 100084 China |
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| 基金项目： | 国家重点基础研究发展计划(973计划) |
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| 摘 要： | Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) scaffolds were prepared by thermally inducing phase separation (TIPS) for bone reconstruction. Scanning electron microscopy and porosity measurements were used to analyze the structure and properties of the scaffolds. The pore diameter of the scaffolds could be easily controlled by changing the quenching temperature and time. The biocompatibility was assessed by examining the proliferation and morphology of MC 3T3-E1 osteoprogenitor cells seeded on the scaffolds. Cultures grown in the presence of a source of phosphate ions showed the formation of a mineralized extracellular matrix. The results indicate that PHBHHx scaffolds prepared using TIPS are a promising candidate for bone reconstruction.
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| 关 键 词： | 聚乙烯 成骨细胞 骨头重建 医疗方法 |
| 收稿时间： | 2006-05-20 |
| 修稿时间： | 2006-05-20 |
Effects of Quenching Temperature and Time on Pore Diameter of Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) Porous Scaffolds and MC3T3-E1 Osteoblast Response to the Scaffolds |
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| XI Jing,LI Jing,ZHU Lin,GONG Yandao,ZHAO Nanming,ZHANG Xiufang. Effects of Quenching Temperature and Time on Pore Diameter of Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) Porous Scaffolds and MC3T3-E1 Osteoblast Response to the Scaffolds[J]. Tsinghua Science and Technology, 2007, 12(4): 366-371. DOI: 10.1016/S1007-0214(07)70055-X |
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| Authors: | XI Jing LI Jing ZHU Lin GONG Yandao ZHAO Nanming ZHANG Xiufang |
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| Affiliation: | State Key Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China |
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| Abstract: | Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) scaffolds were prepared by thermally inducing phase separation (TIPS) for bone reconstruction. Scanning electron microscopy and porosity measurements were used to analyze the structure and properties of the scaffolds. The pore diameter of the scaffolds could be easily controlled by changing the quenching temperature and time. The biocompatibility was assessed by examining the proliferation and morphology of MC 3T3-E1 osteoprogenitor cells seeded on the scaffolds. Cultures grown in the presence of a source of phosphate ions showed the formation of a mineralized extracellular matrix. The results indicate that PHBHHx scaffolds prepared using TIPS are a promising candidate for bone reconstruction. |
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| Keywords: | poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) MC 3T3-E1 osteoblast polymer scaffold bone reconstruction |
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