| 柞蚕抗菌蛋白纯化及性质 |
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| 引用本文： | 赵小凡,王金星,王绪英,齐红英,邓怿.柞蚕抗菌蛋白纯化及性质[J].山东大学学报(理学版),1999,34(3):5. |
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| 作者姓名： | 赵小凡 王金星 王绪英 齐红英 邓怿 |
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| 作者单位： | 山东大学生命科学院 |
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| 摘 要： | 从雄性柞蚕（ Ａｎｔｈｅｒａｅａ ｐｅｒｎｙｉ） 蛹中分离和纯化抗菌蛋白．该抗菌蛋白由大肠杆菌诱导产生，经过 Ｓｅｐｈａｄｅｘ Ｇ５０ 分离纯化，在 Ｓ Ｄ Ｓ Ｐ Ａ Ｇ Ｅ 检测中表现为一条带，分子量在１５ ｋ Ｄａ 左右．纯化产物对大肠杆菌有明显抗菌活性，对血细胞没有凝集作用，是抗菌蛋白．
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| 关 键 词： | 柞蚕 抗菌蛋白 凝集作用 |
PURIFICATION AND CHARACTERIZATION
OF AN
ANTIPROTEIN FROM ANTHERAEA PERNYI |
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| Zhao Xiaofan,Wang Jinxing,Wang Xuying,Qi Hongying,Deng Yi.PURIFICATION AND CHARACTERIZATION
OF AN
ANTIPROTEIN FROM ANTHERAEA PERNYI[J].Journal of Shandong University,1999,34(3):5. |
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| Authors: | Zhao Xiaofan Wang Jinxing Wang Xuying Qi Hongying Deng Yi |
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| Abstract: | The purification and Characterization of an antiprotein from male pupae of Antheraea pernyi are reported here.This antiprotein is induced by injection of Escherichia coli in the abdomen of pupae.The antiprotein is purified by chromatography of Sephadex G 50.The purified protein appears almost homogeneous and the molecular mass is estimated as 15 kDa on SDS PAGE. The purified protein is capable of antibacterial but no activity of coagulating red cells,which suggested that the protein is not a lectin.The mechanism of antibacterial of this protein is remained to be clarified. |
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| Keywords: | antheraea pernyi antiprotein coagulating action |
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