| T载体的应用及研究进展 |
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| 引用本文： | 田生礼,梁秀怡,梁志成. T载体的应用及研究进展[J]. 科学技术与工程, 2014, 14(30) |
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| 作者姓名： | 田生礼 梁秀怡 梁志成 |
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| 作者单位： | 1. 深圳大学生命科学学院深圳市微生物基因工程重点实验室,深圳,518060
2. 深圳大学生命科学学院深圳市海洋生物资源与生态环境重点实验室,深圳,518060 |
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| 摘 要： | T载体可直接用于克隆PCR产物,因此应用于大规模的基因克隆中,具有快速、高效、操作简单等优点。对T载体进行了综述,介绍了T载体在生物学众多领域的广泛应用,总结了当今T载体的技术创新和改良,特别介绍了目前最新的新型多功能T载体——定向克隆表达型T载体,并且探讨了T载体未来的发展趋势。
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| 关 键 词： | T载体 TA克隆 定向克隆 表达型T载体 |
| 收稿时间： | 2014-06-03 |
| 修稿时间： | 2014-06-26 |
Research and Application of T vector |
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| TIAN Sheng-li,LIANG Xiu-yi and LIANG Zhi-cheng. Research and Application of T vector[J]. Science Technology and Engineering, 2014, 14(30) |
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| Authors: | TIAN Sheng-li LIANG Xiu-yi LIANG Zhi-cheng |
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| Affiliation: | College of Life Sciences, Shenzhen Key Laboratory of Microbial Genetic Engineering, Shenzhen University,College of Life Sciences, Shenzhen Key Laboratory of Marine Bioresources and Ecology, Shenzhen University, |
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| Abstract: | T vector can be directly used for cloning of PCR products, and thus has been used in large-scale gene cloning, with fast, efficient, and easy operation. This paper reviews the T vector widely used in many fields of biology, focuses on the new T vector technology innovation and improvement. The latest multifunctional T vector - directional cloning and expression T vectors are introduced and the future and trends of T vector research are discussed. |
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| Keywords: | T Vector TA Cloning Directional Cloning Expression T vector |
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