Evidence for a novel cytoplasmic processing event in ribosome maturation in the sea urchin Paracentrotus lividus |
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Authors: | Daniele Bellavia Rainer Barbieri |
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Institution: | 1. Dipartimento di Biologia Cellulare e dello Sviluppo, Università degli Studi di Palermo, V.le delle Scienze, Edificio 16, 90128, Palermo, Italy
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Abstract: | In this paper we demonstrate the existence of a cytoplasmic processing step, never before described, involving both the pre-ribosomal
subunits in the sea urchin Paracentrotus lividus. Northern-blot hybridization, primer extension, S1 mapping experiments and in situ hybridizations allowed us to demonstrate
that cytoplasmic processed particles are successively re-imported into the nucleus where maturation of their RNAs is completed
prior to being exported to the cytoplasm. Our findings lead to the proposal of a new model of ribosome maturation and shuttling. |
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Keywords: | |
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