几种物理因素对黄芩细胞悬浮系生长与黄芩苷含量变化的影响

为建立稳定的黄芩细胞悬浮系,采用悬浮细胞培养技术,研究了接种量、光照与否和pH值等3种物理因素对黄芩细胞悬浮系生长与黄芩苷含量变化的影响。结果表明,黄芩细胞悬浮系浊度随培养时间呈现"S"形变化曲线;采用MS基本培养基,添加0.2 mg·L-1 2,4-D、2.0 mg·L-1 6-BA和3%蔗糖,选取1.0 g·L-1的接种量、pH 5.8,并在25℃条件下进行暗培养,最有利于黄芩苷的积累,黄芩苷含量最高可达7.425μg·g-1FW。

Effects of some physiocal factors on growth of cell suspension culture from Scutellaria baicalins and changes of baicalin content

For establish stable cell suspension skullcap,cell culture technique were used to study the three kinds of physical factors such as inoculation,light and pH on change of growth of Scutellaria suspension cell lines and the baicalin content. The results showed that the turbidity of skullcap cell suspension presented＂S＂shaped curve with incubation time. Using MS basic medium , add 0.2 mg · L-1 2,4-D, 2.0 mg · L-1 6-BA and 3 % sucrose , 1.0g·L-1 of inoculum,pH5.8,and incubated at 25℃dark conditions most conducive to the accumulation of baicalin, and baicalin up to 7.425μg·g-1FW.