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Visualization of protein RecR in Escherichia coli by fluorescent labelling
Authors:Jiefang Qiu  Xuefeng Pan
Institution:School of Life Science, Beijing Institute of Technology, Beijing 100081, China
Abstract:RecR protein, a functional equivalent of Rad52 in eukaryotes, plays a critical role in the RecF pathway of homologous recombination in Escherichia coli. By constructing and expressing the recR-yfp hybrid gene, the distribution of the RecR-YFP fusion protein was visualized in E. coli by fluorescent microscopy. Our results showed that RecR proteins can be localized predominantly in the nucleoid region of E. coli. By measuring the UV resistance of a recR mutant carrying the recR-yfp gene in the plasmid, the expressed RecR-YFP was found to be functional in improving the UV resistance of the recR deficiency strain.
Keywords:RecR  YFP  DNA Repair  Homologous recombination  E  coli
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