| 五针松针叶愈伤组织的诱导和培养基优化 |
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| 引用本文： | 张德炎,李建民.五针松针叶愈伤组织的诱导和培养基优化[J].青海师范大学学报(自然科学版),1997(3):55-59. |
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| 作者姓名： | 张德炎 李建民 |
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| 作者单位： | [1]湖北省咸宁教育学院 [2]青海师范大学 |
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| 基金项目： | 国家教委重点跟踪年轻教师基金 |
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| 摘 要： | 以五针松针叶切段为外植体，接种在ＧＤ和ＭＢ二种基本培养基上进行比较实验。结果表明，ＧＤ培养基诱导五针松针叶愈伤组织效果较好。在此基础上，对２，４－Ｄ，ＢＡ，肌醇，ＣＨ和蔗糖五个因子，四种水平进行了正交设计试验，试验结果表明，五针松针叶愈伤组织生长的最佳培养基配方和为ＧＤ＋５ｍｇ／Ｌ２，４－Ｄ＋１ｍｇ／ＬＢＡ＋５００ｍｇ／Ｌ肌醇＋６００ｍｇ／ＬＣＨ＋３％蔗糖＋０．７％琼脂。
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| 关 键 词： | 五针松 地叶 愈伤组织 GD培养基 优化 |
Inducement of Calli from Pinus strobus and Optimization of the Medium |
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| Zhang Deyan.Inducement of Calli from Pinus strobus and Optimization of the Medium[J].Journal of Qinghai Normal University(Natural Science Edition),1997(3):55-59. |
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| Authors: | Zhang Deyan |
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| Institution: | Zhang Deyan(Xianning Deucation Colloege)Li Jianmin; Li Xiwen; Xing Miao(Qinghai Normal University) (Northeast Normal University) |
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| Abstract: | Needle leaves of Pinus strobus were cut into sections and cultured on GD and MB media,respectively. Results showed that the GD medium was better than MB one in terms of inducingcalli. The optimum composition of the medium for the growth of the calli was screened by usingorthogonal design test which included five different factors and four levels. The result of the testsuggests that the optimum calli growth medium is GD +5mg/L2, 4-D+ 1mg/L BA +500mg/L Inositol + 600mg/L CH + 3 % Sucrose +0. 7%Agar. |
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| Keywords: | Pinus Strobus Needle leave calli GD medium |
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