Microdissection and amplification of the chromosome arm 5S in a rice telo-tetrasomic |
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Authors: | Zhukuan Cheng Huihuanq Yan Benyuan Dang Zanmin Hu Minghong Gu Lihuang Zhu |
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Institution: | (1) Institute of Genetics, Chinese Academy of Sciences, 100101 Beijing, China;(2) Agricultural College, Yangzhou University, 225009 Yangzhou, China |
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Abstract: | The rice aneuploids with telochromosomes are ideal genetic stocks for chromosome arm identification and microdissection. A
rice telo-tetrasomic line with two extra short arms of chromosome 5 (5S) was used in the present study. The amrs of 5S were
microdissected from the prometaphase cells in mitosis and amplified with linker adaptor PCR (LA-PCR). The amplified fmgments,
ranging from 200–3000 bp, were confirmed to be from the rice chromosome arm 5S by the rice STS and miamatellite markers. |
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Keywords: | telo-tetrasomic chromosome arm microdissection rice |
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