Genetic analysis and gene fine mapping for a rice novel mutant （rl9〈t）） with rolling leaf character

Much attention has been paid to leaf shape of rice in the process of ideotype breeding1]. Several authors have reported that the rolling of leaf in some degree helps keep it erect, consequently optimizing canopy light transmission condition, which is good for dry matter accumulation and for high yield2―6]. Rice as a polymorphic crop has many types of vari- ety with different morphologies. In terms of leaf shape, different cultivars with rolling leaf have been identifiedin rice germplasm. Le…

Genetic analysis and gene fine mapping for a rice novel mutant (rl 9(t) ) with rolling leaf character

Leaf shape is an important parameter for ideotype breeding in rice, and the rolling of leaf is also beneficial to efficient ripening of grains. This encourages the explorations of new genes that regulate leaf shape. In this study, genetic analysis and gene mapping were carried out for a novel rolling leaf mutant identified from japonica variety Zhonghua 11. The SSR marker analysis showed that the mutant was controlled by a single recessive gene (rl9(t)) lo- cated on chromosome 9. Fine mapping of the Rl9(t) locus was conducted with 30 new STS markers de- veloped around Rl9(t) anchored region based on the sequence diversity between Nipponbare and 93-11. The fine mapping necessitated the contruction of a PAC contig encompassing the Rl9(t) locus, which was delimited to a 42 kb region. This could therefore en- hance the cloning of the target gene in further stud- ies.