SD大鼠肝癌演进中端粒酶活性的动态变化

探讨端粒酶活性在肝癌演进中的变化,用3'-甲基-4-二甲基氨基偶氮苯(3'-Me-DAB)作诱变剂,建立SD大鼠肝癌变动物模型.自灌胃之日起,分别在2、4、6、8、10、12、14、17w分批处死大鼠,取其肝脏,进行病理分级,然后用TRAP-ELISA法检测肝癌变各时期端粒酶活性,结果显示:在轻度炎性反应期(LI)端粒酶活性高于对照组,呈极显著差异(P<0.01);重度炎性反应期(HI)端粒酶活性有所下降,但仍高于对照组,且呈显著性差异(P<0.05);至腺瘤样增生(NH)和肝内胆管细胞癌(CCC)时端粒酶活性再次升高,与对照组相比呈极显著差异(P<0.01).提示端粒酶活性增高可能是肝癌变进程中的重要事件,与肝癌的发生发展密切相关.

Dynamic Changes of Telomerase during the Hepatocarcinogenesis in SD Rats

To discuss the dynamic changes of telomerase during the hepatocarcinogenesis in SD rats,animal models of every pathologic stage are established by 3’-methyl-4-dimethylaminoazobenzene(3’-Me-DAB) gavage.Animals are sacrificed respectively at 2,4,6,8,10,12,14 and 17 weeks from the first day of gavage and the livers are harvested.After pathological grading,TRAP-ELISA has been used to explore the changes of telomerase in the whole process of carcinogenesis.Results show the following: In light inflammation(LI),the expression of telomerase is higher and the difference is significant compared with the control group(P<0.01);To heavy inflammation(HI),the expression of telomerase is gradually decreased but is still higher than the control group(P<0.05);To nodus hyperplasia(NH) and cholangiocellular carcinoma(CCC),the expression of telomerase gradually increase again and there are significant differences between the induced and the control group(P<0.01).The result suggests that the increased activity of telomerase may be very important in hepatocarcinogenesis,so telomerase is closely related to the development of liver cancer.