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核黄素诱导番茄幼苗抗白粉菌机理研究
引用本文:裴冬丽,丁锦平,张庆琛,王良元,李成伟. 核黄素诱导番茄幼苗抗白粉菌机理研究[J]. 河南师范大学学报(自然科学版), 2012, 40(5): 121-124
作者姓名:裴冬丽  丁锦平  张庆琛  王良元  李成伟
作者单位:1. 商丘师范学院生命科学学院,植物与微生物互作重点实验室,河南商丘476000
2. 商丘师范学院生命科学学院,植物与微生物互作重点实验室,河南商丘476000;周口师范学院生命科学学院;植物遗传与分子育种重点实验室,河南周口466001
基金项目:国家自然科学基金(31071807);河南省科技厅基础与前沿技术项目(122300410226)
摘    要:研究了2mmol/L核黄素诱导感病番茄品种Moneymaker对白粉菌(Oidium neolycopersici)的抗性及相关生理反应:叶片H2O2积累、丙二醛(malonaldehyde,MDA)含量、超氧阴离子(O2-)产生速率和苯丙氨酸解氨酶(phenylalanine ammonialyase,PAL)、过氧化物酶(peroxidase,POD)活性的变化.核黄素诱导后番茄MM对O.ne-olycopersici的抗性增强,诱发了过敏反应(hypersensitive response,HR),番茄叶片内MDA的含量、O2-产生速率、PAL,POD活性均高于核黄素未处理,其变化趋势与抗病番茄品种G1.1560与白粉菌组成的非亲和组合相似.结果表明,H2O2的积累、膜脂过氧化、O2-,PAL和POD在核黄素诱导感病番茄对白粉菌的抗性反应中发挥重要作用.

关 键 词:核黄素  番茄  白粉菌  诱导抗性  防御反应

Riboflavin Induced Tomato Resistance to Powdery Mildew
PEI Dong-li , DING Jin-ping , ZHANG Qing-chen , WANG Liang-yuan , LI Cheng-wei. Riboflavin Induced Tomato Resistance to Powdery Mildew[J]. Journal of Henan Normal University(Natural Science), 2012, 40(5): 121-124
Authors:PEI Dong-li    DING Jin-ping    ZHANG Qing-chen    WANG Liang-yuan    LI Cheng-wei
Affiliation:1,2(1.Key Laboratory of Plant-Microbe Interactions,Department of Life Science,Shangqiu Normal University,Shangqiu 47600,China;2.Key Laboratory of Plant Genetics and Molecular Breeding,Department of Life Scicence,Zhoukou Normal University,Zhoukou 466001,China)
Abstract:It was studied that 2 mmol/L riboflavin induced changes of leaves H2O2 accumulation,MDA content,O-2 production rate and activity of PAL and POD in interaction of susceptive tomato MM with Oidium neolycopersici,and the effects of riboflavin on tomato resistance to powdery mildew.It was shown that treatment of riboflavin increased the MM resistance to O.neolycopersici,induced HR in MM,H2O2 accumulation,MDA content,O-2 production rate and POD、PAL activity in tomato leaves were higher than control of no riboflavin treatment,of which the change tendency was generally similar to that of incompatible interaction of highly resistant tomato G1.1560 with O.neolycopersici.The results suggested that riboflavin induced physiological changes including H2O2 accumulation,lipid peroxidation,O-2 production,POD and PAL could play a role in the increase of tomato resistance to O.neolycopersici.
Keywords:riboflavin  tomato  O.neolycopersici  induced resistance  defense response
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