A tumor suppressor genefhit prokaryotic expression and identification |
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Authors: | Sun Yan Li Wen-xin Huang Zan Jiang Da-he |
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Institution: | (1) College of life sciences, Wuhan University, 430072 Wuhan |
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Abstract: | The recombinant expression vector pGEMD-fhit which contains full encoding region offhit gene was constructed. The recombinant was introduced into the BL21 (DE3) strain ofE. coli and induced by 1 mmol/L IPTG to express a 29×103 polypeptide offhit fusion protein. And the 29×103 protein was sensitive and specific in reaction with anti-fhit antibody in Western blot.
Foundation item: Supported by the National Natural Science Foundation of China (39770373)
Biography: SUN Yan (1975−), female, Master of science, Research direction: gene engineering |
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Keywords: | fhit gene expression western blot |
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