香烟烟雾提取物对人内皮细胞细胞色素C氧化酶活性及细胞凋亡的影响

目的探讨香烟烟雾提取物（cigarette smoking extract，CSE）对内皮细胞细胞色素C氧化酶（cytochmme Coxidase，COX）活性及凋亡的影响。方法体外培养ECV304，分别给予0％、0．5％、1％、5％CSE刺激12h，及5％CSE刺激0h、6h、12h、24h后，生化法检测COX活性；投射电镜和流式细胞仪观察细胞凋亡情况。结果CSE引起COX活性下降，且随着刺激浓度和时间的增加而下降（P〈0．05）；电镜示CSE干预组细胞出现明显的凋亡形态学改变；流式细胞仪结果示不同浓度CSE分别作用12h后凋亡率依次增高，除O％CSE组和0．5％CSE组间比较无统计学意义（P〉0．05），余各组间比较均有统计学意义（P〈0．05）；5％CSE作用不同时间后，随着干预时间的延长细胞凋亡率逐渐升高（P〈0．05）。结论CSE抑制内皮细胞COX活性，呈浓度和时间依赖性；CSE诱导内皮细胞凋亡，呈浓度和时间依赖性；COX活性的下降可能在CSE所致的内皮细胞凋亡中具有重要作用。

Effect of Cigarette Smoking Extract on the Activity of Cytochrome C Oxidase and Apoptosis in Human Endothelial Cells

Objective To investigate the effect of cigarette smoking extract (CSE) on the activity of cytochrome C oxidase (COX) and apoptosis of human endothelial cells. Methods: After ECV304 were treated with CSE, COX activity was detected by biochemistry; CSE-induced cell apoptosis was observed by Flow Cytometry; Cell morphology of apoptosis was observed under a transmission electron microscope. Results: CSE decreased the value of COX activity (all P<0.05) ,CSE induced apoptosis of the cells. The effects of CSE on ECV304 were all in a doseand-time dependent manner. Morphological observation indicated that CSE induced characteristic apoptotic changes in ECV304. Conclusion:CSE inhibits the activity of COX and induces apoptosis of endothelial cells in time-and concentration-dependent manner. The decrease of COX activity may play an important role in CSE-induced apoptosis of endothelial cells.