Heterogeneous bioenergetic behaviour of subsarcolemmal and intermyofibrillar mitochondria in fed and fasted rats |
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Authors: | M P Mollica L Lionetti R Crescenzo E D’Andrea M Ferraro G Liverini S Iossa |
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Institution: | (1) Dipartimento delle Scienze Biologiche, Sezione di Fisiologia, Via Mezzocannone 8, 80134 Napoli, Italy |
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Abstract: | This study was designed to examine energetic behaviour of skeletal muscle subsarcolemmal and intermyofibrillar mitochondrial
populations. The data show that subsarcolemmal mitochondria exhibited a lower degree of coupling and efficiency than intermyofibrillar
ones, and can therefore be considered less efficient at producing ATP. In addition, subsarcolemmal mitochondria showed an
increased sensitivity to palmitate-induced uncoupling, in line with high adenine nucleotide translocator content and decreased
oxidative damage. We then determined the effect of 24 h fasting on energetic characteristics of skeletal muscle mitochondrial
populations. We found that fasting enhanced proton leak and decreased the degree of coupling and efficiency, both in the absence
and in the presence of palmitate only in subsarcolemmal mitochondria. Moreover, this mitochondrial population showed lower
oxidative damage, probably due to a counter-regulatory mechanism mediated by uncoupling protein 3. Subsarcolemmal and intermyofibrillar
mitochondria appear to exhibit different energetic characteristics and can be differently affected by physiological stimuli.
Received 28 September 2005; received after revision 9 November 2005; accepted 28 November 2005 |
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Keywords: | Subsarcolemmal mitochondria intermyofibrillar mitochondria mitochondrial efficiency palmitate-induced proton leak oxidative damage fed-fasting transition |
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