| Efficient expression of human factor Ⅸ cDNA in liver mediated by hydrodynamics-based plasmid administration |
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| 作者姓名： | HE Chenxia FENG Dengmin WU Wenjun DING Youfa CHEN Li CHEN Haoming YAO Jihua SHEN Qi LU Daru & XUE Jinglun . State Key Laboratory of Genetic Engineering Institute of Genetics School of Life Sciences Fudan University Shanghai China . Changhai Hospital The Second Military Medical University Shang-hai China . Hospital of Lishui Zhejiang China Correspondence should be addressed to Xue Jinglun |
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| 作者单位： | HE Chenxia,FENG Dengmin,CHEN Li,CHEN Haoming,YAO Jihua,SHEN Qi,LU Daru,XUE Jinglun(State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, China);Wu Wenjun(Changhai Hospital, The Second Military Medical University, Shanghai 200433, China);DING Youfa(Hospital of Lishui, Zhejiang 323000, China) |
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| 基金项目： | 国家高技术研究发展计划(863计划)，国家自然科学基金，高等学校全国优秀博士学位论文作者专项基金，教育部霍英东教育基金，Award Project for Youth Teachers in High School |
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| 摘 要： | The transfection of plasmid DNA into mammalian cells is an indispensable tool in the study of gene transfer and gene function. Since the original report in 1990 on the successful expression of a reporter gene in muscle1], plasmid DNA injection has been widely used to mediate gene transfer study. As a gene transfer vector, naked DNA has many obvious advantages. It is easy and cheap to be prepared and more safe after transfection. But the plasmid mediated gene expression is low because of t…
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| 关 键 词： | hFⅨ gene transfer naked plasmid hydrodynamics- based transfection. |
Efficient expression of human factor Ⅸ cDNA in livermediated by hydrodynamics-based plasmid administration |
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| HE Chenxia,FENG Dengmin,WU Wenjun,DING Youfa,CHEN Li,CHEN Haoming,YAO Jihua,SHEN Qi,LU Daru & XUE Jinglun . State Key Laboratory of Genetic Engineering,Institute of Genetics,School of Life Sciences,Fudan University,Shanghai ,China, . Changhai Hospital,The Second Military Medical University,Shang-hai ,China, . Hospital of Lishui,Zhejiang ,China Correspondence should be addressed to Xue Jinglun.Efficient expression of human factor Ⅸ cDNA in livermediated by hydrodynamics-based plasmid administration[J].Chinese Science Bulletin,2003,48(8). |
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| Authors: | HE Chenxia FENG Dengmin Wu Wenjun DING Youfa CHEN Li CHEN Haoming YAO Jihua SHEN Qi LU Daru XUE Jinglun |
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| Institution: | e-mail: jlxue@ fudan.ac.cn |
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| Abstract: | |
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| Keywords: | gene transfer naked plasmid hydrodynamics- based transfection |
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