| Bacillus velenzensis S3-1基因组中表面活性素、伊枯草菌素和丰原素合成基因簇的定位与分析 |
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| 引用本文： | 金清,张蕾,蒋秋悦,苏翠珠,肖明. Bacillus velenzensis S3-1基因组中表面活性素、伊枯草菌素和丰原素合成基因簇的定位与分析[J]. 上海师范大学学报(自然科学版), 2018, 47(4): 451-457 |
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| 作者姓名： | 金清 张蕾 蒋秋悦 苏翠珠 肖明 |
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| 作者单位： | 上海师范大学生命与环境科学学院;农业部都市农业重点实验室 |
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| 基金项目： | 上海市2016年度"科技创新行动计划"项目（16391902100）;农业部都市农业重点实验室开放基金（UA201705） |
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| 摘 要： | 通过Gen Bank数据库寻找能产生表面活性素、伊枯草菌素或丰原素并在基因组中其合成基因已得到注释的芽胞杆菌,经过BLAST比对序列初步确定这三类脂肽类抗菌肽的合成基因簇的位置,再运用anti SMASH进行其合成基因簇的最终定位.对表面活性素、伊枯草菌素和丰原素的结构及相关的合成酶结构域进行了预测与分析.
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| 关 键 词： | 生物信息学 Bacillus velezensis S3-1 表面活性素 伊枯草菌素 丰原素 |
| 收稿时间： | 2018-02-07 |
Localization and analysis of synthetic gene clusters of Bacillus velenzensis S3-1 in surfactin,iturin and fengycin |
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| Jin Qing,Zhang Lei,Jiang Qiuyue,Su Cuizhu and Xiao Ming. Localization and analysis of synthetic gene clusters of Bacillus velenzensis S3-1 in surfactin,iturin and fengycin[J]. Journal of Shanghai Normal University(Natural Sciences), 2018, 47(4): 451-457 |
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| Authors: | Jin Qing Zhang Lei Jiang Qiuyue Su Cuizhu Xiao Ming |
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| Affiliation: | College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China,College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China,College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China,College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China and College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China;Key Laboratory of Urban Agriculture, Ministry of Agriculture, Shanghai 200240, China |
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| Abstract: | In this study,the GenBank database was used to search for Bacillus which could produce surfactin,iturin or fengycin and its synthetic genes were annotated in the genome.The location of the synthetic gene clusters of these three lipopeptide antibiotics were determined by BLAST alignment.Then antiSMASH was used to determine the final location of the synthetic gene clusters.Further,we predicted and analyzed the structure and related synthetase domains of the surfactin,iturin and fengycin. |
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| Keywords: | bioinformatics Bacillus velezensis S3-1 surfactin iturin fengycin |
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