| 珠鸡mtDNA的限制性图谱 |
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| 引用本文： | 郑文竹,洪夏.珠鸡mtDNA的限制性图谱[J].厦门大学学报(自然科学版),2002,41(1):112-116. |
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| 作者姓名： | 郑文竹 洪夏 |
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| 作者单位： | 厦门大学生命科学学院,福建,厦门,361005 |
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| 摘 要： | 应用AvaⅠ，BamHⅠ,BglⅠ,DraⅠ,EcoRⅠ,HpaⅠ,PvuⅡ,SacⅠ,SalⅠ,ScaⅠ和StuⅠ共11种限制酶对珠鸡（Acryllium vulturinum)mtDNA进行单酶切分析，结果表明，珠鸡mtDNA的分子量为16.7kb，另以其中除AvaⅠ和StuⅠ外的9种限制酶进行双酶切分析，构建了珠鸡mtDNA的限制性图谱，并与家鸡（Gallus gallus domesticus)比较，二者mtDNA序列歧异值为0.073。
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| 关 键 词： | 珠鸡 mtDNA 限制性图谱 单酶切分析 分子量 歧异值 酶切位点数 |
| 文章编号： | 0438-0479(2002)01-0112-05 |
| 修稿时间： | 2001年3月9日 |
Mitochondrial DNA Restriction Maps of Acryllium vulturinum |
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| ZHENG Wen-zhu,HONG Xia.Mitochondrial DNA Restriction Maps of Acryllium vulturinum[J].Journal of Xiamen University(Natural Science),2002,41(1):112-116. |
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| Authors: | ZHENG Wen-zhu HONG Xia |
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| Abstract: | Mitochondrial DNA (mtDNA) from Acryllium vulturinum was purified, and then digested with 11 restriction endonucleases ( Ava I, BamH I, Bgl I, Dra I, EcoR I, Hpa I, Pvu II, Sac I, Sal I, Sca I, Stu I ). The results showed that its molecular weight was 16.7 kb.From the comparison of mtDNA between Acryllium vulturinum and Gallus gallus domesticus,the sequence divergence value was 0.073. Furthermore, restriction maps of the mtDNA were obtained with the double digestion method. |
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| Keywords: | Acryllium vulturinum mitochondrial DNA restriction map |
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