Cytolysis through cells dependent on immunization against cellular antigens: identification and enumeration of cytotoxic cells]

In this report we show how to identify and enumerate alloantigen-dependent killer cells. Individual effector cells are associated by micromanipulation to target cells and the doublets are incubated at 37 degrees C. Killers are identified by the lysis of the associated target. Killer cells can be subdivided into two subpopulations: cell responsible for the lysis of the specific target cells which bear the immunizing H-2 antigens and cells which kill only lectin pretreated targets.