水稻稻瘟病菌粗毒素对水稻幼穗组织培养的影响及抗性筛选

利用目前稻瘟病不同的优势小种菌株的液体培养提取的粗毒素对水稻幼穗进行处理,稻瘟病菌粗毒素对幼穗的愈伤诱导和分化有强烈的破坏作用,并且随着处理时间的增加或毒素浓度的增大受到破坏的程度愈深。用稻瘟病菌粗毒素浸泡幼穗对其愈伤诱导和分化都产生很强的抑制作用,能够起到抗性筛选的目的,处理的时间在24h以内可以得到胚性愈伤和分化成苗;筛选时毒素的浓度以30%为宜;用粗毒素直接浸泡幼穗愈伤组织对愈伤的伤害很大,得到的分化植株相对较少,但仍可作为一种抗性愈伤筛选的方法,其处理时间以24h较好;而且不同的水稻品种产生的耐性不同,并可以同时筛选出抗稻瘟病的突变体。

Effect of Crude Toxin from the Pyricularia Oryzae on the Tissure Culture of Young Panicles Rice and the Screening of Anti-Blast Mutant

The growth of the young head induction,callus subculture and differentiation were strong.Inhibited by the crude toxin filtration,contrasted with CK,the results were significant different,and the result was different with toxin concentration changing.With the increasing of the toxin concentration and the prolonging the impact time,the inhibiting was stronger.30% was the better toxin concentration when screening of rice blast-resistant mutant from the mature embryo culture;mutant was hurt strongly,when it was immersed directly in the toxin,and the differentiation seeding was got little,but this methods still can be used to screening of resistant mutant,24h was the apposite time.