Contradictory differential staining results with Coomassie Brilliant Blue and silver carbonate on sister chromatids |
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| Authors: | K Y Jan Sheng Wang-Wuu Wu-Nan Wen |
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| Institution: | (1) Institute of Zoology, Academic Sinica, 115 Taipei, Taiwan;(2) Institute of Biochemistry, College of Medicine, National Taiwan University, 100 Taipei, Taiwan |
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| Abstract: | Summary Coomassie Brilliant Blue, and silver stain, are used in electrophoretic gels to identify polypeptides. The relative staining intensity has been taken as indicating a quantitative difference between proteins. However, when these 2 stains were applied to 2 identically pretreated chromosome preparations, contradictory staining results were obtained.Acknowledgment. This work is supported under the auspices of the National Science Council, Republic of China. |
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