| 重组大肠杆菌高密度发酵表达胰高血糖素样肽-1衍生物(GP62)的培养基优化 |
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| 引用本文： | 蒋德旗,周天鸿,冉艳红,陈英,桑延霞,李弘剑.重组大肠杆菌高密度发酵表达胰高血糖素样肽-1衍生物(GP62)的培养基优化[J].暨南大学学报,2011,32(3):334-338. |
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| 作者姓名： | 蒋德旗 周天鸿 冉艳红 陈英 桑延霞 李弘剑 |
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| 作者单位： | 暨南大学生命科学技术学院生物工程学系,广东广州,510632 |
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| 基金项目： | the Ministry of Science and Technology of China(2009ZX09103-606); Guangzhou Scientific and Technology Commission(2009Z1-E151) |
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| 摘 要： | 对重组E.coli BL21 (DE3)高密度表达胰高血糖素样肽-1衍生物(GP62)的发酵培养基进行了优化.通过单因素实验与正交实验相结合的方法,依次对基础培养基,发酵培养基中碳源、氮源、无机盐等成份进行优化.在2YT培养基基础上,获得优化的发酵培养基2YT-GP62.在5L全自动发酵罐中利用终浓度0.5 mmol/...
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| 关 键 词： | 大肠杆菌 培养基 IPTG GLP-1 |
High cell-density production of glucagon-like peptide-1 derivate(GP62) in recombinant E.coli by optimization of culture medium |
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| JIANG De-qi,ZHOU Tian-hong,RAN Yan-hong,CHEN Ying,SANG Yan-xia,LI Hong-jian.High cell-density production of glucagon-like peptide-1 derivate(GP62) in recombinant E.coli by optimization of culture medium[J].Journal of Jinan University(Natural Science & Medicine Edition),2011,32(3):334-338. |
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| Authors: | JIANG De-qi ZHOU Tian-hong RAN Yan-hong CHEN Ying SANG Yan-xia LI Hong-jian |
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| Institution: | JIANG De-qi,ZHOU Tian-hong,RAN Yan-hong,CHEN Ying,SANG Yan-xia,LI Hong-jian(Department of Bioengineering,College of Life Science and Technology,Jinan University,Guangzhou 510632,China) |
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| Abstract: | This study aims to get high expression level of GLP-1 derivate(GP62) in high cell-density recombinant Escherichia coli BL21(DE3) by optimizing its fed-batch culture medium.The effects of different basal medium,yeast extract and inorganic salt concentration on cell growth and expression of GLP-1 derivate(GP62) in recombinant E.coli BL21(DE3) were investigated by single factor test and orthogonal array.Optimized 2YT-GP62 medium was obtained by improving the 2YT medium.The highest productivity of 4.40 mg fusio... |
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| Keywords: | Escherichia coli culture medium IPTG Glucagon-like peptide-1 |
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