裸鼠自发性淋巴道转移肿瘤模型的建立

在裸鼠发现一例自发性皮下肿瘤,伴广泛淋巴结转移,经10代以上体内传代,在裸鼠体内长瘤及转移的特性稳定,原位长瘤率大于95％,潜伏期5—12天,转移率100％,平均荷瘤生存时间22天。大体解剖见腹股沟、腋下、颌下、腹腔等处有不同程度淋巴结转移。光镜和电镜的形态学检查见肿瘤细胞形态圆、较小、核大胞浆少,染色质呈块状、围裙状,分裂相多见,符合小细胞分化程度较差的恶性肿瘤,转移灶和原位灶形态学上相似。该可稳定传代、广泛淋巴道转移的小鼠肿瘤定名为LMLM瘤系。

A MODEL OF SPONTANEOUS TUMOR WITH LYMPHATIC METASTASIS IN NUDE MOUSE

Spontaneous tumor nodes were discovered by accident in a nude mouse (BA-BL/c nu/nu). These nodes were implanted subcutaneously with more than ten passages in more than one hundred nude mice. The primary and metastatic tumor nodes were observed by means of anatomy, light and electronic microscopy. Results showed that this tumor (LMLM) was a murine tumor with widespread lymph node metastasis which could be transplanted stably. Tumor appeared on the 5th to 15th days after transplantation, tumor occurrence rate was more than 95%. Life span of the mice bearing the LMLM tumor was 25 to 35 days after transplantation. There was no visible tumor node in mouse's lungs, liver, stomach, gut, kidneys, spleen, etc. This tumor can grow in the heterozygous nude mouse (BALB/c nu/ ) when implanted subcutaneously.