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| Differential gene expression profiling in the developed ovaries between the parthenogenetic and bisexual female rice water weevils,Lissorhoptrus oryzophilus Kuschel （Coleoptera： Curculionidae） | |
| 作者姓名： | YANG Pu ZHOU WenWu ZHANG Qin CHENG JiaAn ZHU ZengRongx WAY MO |
| 作者单位： | [1]State Key Laboratory of Rice Biology; Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture Institute of Insect Sciences, Zhejiang University, Hangzhou 310029, China [2]Department of Plant Protection, College of Biosafety Science and Technology, Hunan Agricultural University, Changsha 410128 China [3]Texas A ＆ M University Agricultural Research and Extension Center, 1509 Aggie Dr., Beaumont, TX 77713, USA |
| 基金项目： | Supported by the National Key Basic Research and Development Program of China (Grant No. 2009CB119200), National High Technology Research and Development Program of China (Grant No. 2007AA10Z220) and Key Projects in the National Science & Technology Pillar Program in the Eleventh Five-year Plan Period (Grant No. 2006BAD17B06) |
| 摘 要： | The rice water weevil, Lissorhoptrus oryzophilus Kuschel （Coleoptera： Curculionidae）, reproduces by sex in the Southeastern United States, but reproduces by parthenogenesis in California and other invaded regions in Asia and Europe. The objective of this study was to create a parthenogenetic gene expression profile of the rice water weevil in order to gain a better insight into the molecular mechanisms of parthenogenesis in the weevil. Suppression subtractive hybridization （SSH） technique was employed for profiling differential gene expression in the developed ovary between the parthenogenetic and bisexual female rice water weevils. A total of 70 contigs were obtained, and the BLASTX search identified putatively 28 genes with differential functions. According to the cytological process of parthenogenesis, the tubulin alpha-1 chain and signal transduction genes etc. were selected for real time quantitative RT-PCR analyses, and their possible functions related to the molecular mechanism of parthenogenesis were discussed. The tubulin alpha-1 chain and some signal transduction genes may be related to the molecular mechanisms of parthenogenesis of the rice water weevil. |
| 关 键 词： | 基因差异表达 稻水象甲 发达国家 象虫科 鞘翅目 卵巢 女性 抑制性消减杂交 |
| 收稿时间： | 2009-04-01 |
| 修稿时间： | 2009-06-02 |
Differential gene expression profiling in the developed ovaries between the parthenogenetic and bisexual female rice water weevils, Lissorhoptrus oryzophilus Kuschel (Coleoptera: Curculionidae) |
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| YANG Pu,ZHOU WenWu,ZHANG Qin,CHENG JiaAn,ZHU ZengRongx,WAY MO.Differential gene expression profiling in the developed ovaries between the parthenogenetic and bisexual female rice water weevils, Lissorhoptrus oryzophilus Kuschel (Coleoptera: Curculionidae)[J].Chinese Science Bulletin,2009,54(20):3822-3829. | |
| Authors: | Pu Yang WenWu Zhou Qin Zhang JiaAn Cheng ZengRong Zhu and M O Way |
| Institution: | 1 State Key Laboratory of Rice Biology|Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture|Institute of Insect Sciences, Zhejiang University, Hangzhou 310029, China; 2 Department of Plant Protection, College of Biosafety Science and Technology, Hunan Agricultural University, Changsha 410128, China; 3 Texas A &|M University Agricultural Research and Extension Center, 1509 Aggie Dr., Beaumont, TX 77713, USA |
| Abstract: | The rice water weevil, Lissorhoptrus oryzophilus Kuschel (Coleoptera: Curculionidae), reproduces by sex in the Southeastern United States, but reproduces by parthenogenesis in California and other in-vaded regions in Asia and Europe. The objective of this study was to create a parthenogenetic gene expression profile of the rice water weevil in order to gain a better insight into the molecular mecha-nisms of parthenogenesis in the weevil. Suppression subtractive hybridization (SSH) technique was employed for profiling differential gene expression in the developed ovary between the parthenoge-netic and bisexual female rice water weevils. A total of 70 contigs were obtained, and the BLASTX search identified putatively 28 genes with differential functions. According to the cytological process of parthenogenesis, the tubulin alpha-1 chain and signal transduction genes etc. were selected for real time quantitative RT-PCR analyses, and their possible functions related to the molecular mechanism of parthenogenesis were discussed. The tubulin alpha-1 chain and some signal transduction genes may be related to the molecular mechanisms of parthenogenesis of the rice water weevil. |
| Keywords: | Lissorhoptrus oryzophilus Kuschel rice water weevil parthenogenesis expression profiling SSH tubulin signal transduction |
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