温度对透明质酸分批发酵的影响及动力学模型

文章研究了30℃~40℃范围内透明质酸(HA)分批发酵过程,发现最适的细胞生长温度是34℃,而40℃时HA的产量最高为0.810g.L-1。以现有的底物抑制和产物抑制动力学模型为基础,建立HA发酵过程中菌体生长,HA合成,乳酸合成及葡萄糖消耗的动力学模型,并计算出不同温度下相关的动力学参数,在此基础上,进一步研究了温度同细胞生长动力学参数之间的内在联系,结果表明H inshelwood模型能够很好地反应在HA分批发酵过程中细胞浓度的变化同温度以及底物浓度之间的一般关系式。

Kinetic Models for the Effect of Temperature on Batch Hyaluronic Acid Fermentation

The effect of temperature varied from 30℃ to 40℃ on the batch fermentation of HA was studied.It was found that the cell growth was hastened at 34℃ and HA production reached to 0.810g·L-1 under the optimum temperature 40℃.Kinetic parameters of glucose consumption,cell growth and HA production were estimated under different temperature,and the results of parameters were estimated from the model.An equation was developed and applied to interlink the relationship between biomass concentration and temperature,as well as substrate concentratiom in the batch glutatione fermentation.The results show that Hinshelwood model could predict the growth pattern very well.