| 猪Motilin和Ghrelin基因克隆及其真核载体的构建与体外表达 |
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| 引用本文： | 应牡英. 猪Motilin和Ghrelin基因克隆及其真核载体的构建与体外表达[J]. 江西科技师范学院学报, 2006, 0(6): 103-106 |
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| 作者姓名： | 应牡英 |
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| 作者单位： | 华中农业大学生命科学院国家微生物重点实验室,湖北,武汉,430070 |
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| 摘 要： | 目的:克隆Motilin和ghrelin基因,构建表达Motilin的pDsRed-Express-motilin载体和表达ghrelin的pEGFP-ghrelin载体,并检测它们能否在真核细胞中表达及表达量的变化。方法:用RT-PCR方法分别从猪小肠和胃的cDNA上扩Motilin和ghrelin基因片段,并分别克隆到pDsRed-Express-N1载体和pEGFP-N1载体上,用脂质体法转染RIBS2细胞系(来自于肾),并用半定量RT-PCR和荧光成像法检测目的基因的表达。结果:构建了表达Motilin的pDsRed-Express-motilin和表达ghrelin基因的pEGFP-ghrelin载体,并能在真核细胞中超表达目的基因,为进一步相关研究提供了实验基础。
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| 关 键 词： | 胃动素Motilin 生长激素释放肽Ghrelin 转染 RIBS2细胞系 |
| 文章编号： | 1007-3558（2006）06-0103-04 |
| 收稿时间： | 2006-09-10 |
| 修稿时间： | 2006-09-10 |
Cloning of Pig Motilin and Ghrelin,Construction of Recombinant pDsRed-Express-Motilin and pEGFP-Ghrelin Vector and Expression in Vitro |
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| Ying Muying. Cloning of Pig Motilin and Ghrelin,Construction of Recombinant pDsRed-Express-Motilin and pEGFP-Ghrelin Vector and Expression in Vitro[J]. Journal of Jiangxi Science & Technology Normal University, 2006, 0(6): 103-106 |
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| Authors: | Ying Muying |
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| Affiliation: | Central China Agricultural University, Wuhan 430070, P.R. China |
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| Abstract: | Aim: clone big motilin and ghrelin gene,construct recombinant pDsRed-expree-motilin and pEGFP-ghrelin vector and their expressions in vitro.Method: motilin and ghrelin genes were amplified by PCR and were cloned to pDsRed-Express-N1 and pEGFP-N1,respectively.Then the resultant pDsRed-Express-Motilin and pEGFP-ghrelin were co-transferred into RIBS2 cells with lipd2000.Result: construction combinations of pDsRed-Express-motilin and EGFP-ghrelin vectors are successful and their expressions are highly efficient in RIBS2 cells. |
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| Keywords: | Motilin Ghrelin infection RIBS2 cells |
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