| 枯草芽孢杆菌生产鸟苷的途径分析 |
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| 引用本文： | 张蓓,王健,陈宁,张克旭,浅井博,方杰.枯草芽孢杆菌生产鸟苷的途径分析[J].南开大学学报,2005,38(1):6-11. |
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| 作者姓名： | 张蓓 王健 陈宁 张克旭 浅井博 方杰 |
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| 作者单位： | 天津科技大学,生物工程学院,天津,300222;吉林大学,生物与农业工程学院,长春,130012;早稻田大学,理工学部,日本,东京,169-8555 |
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| 基金项目： | Supported by the Science and Technology Development Foundation of Tianjin Colleges and Universities(20031205) |
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| 摘 要： | 利用途径分析的方法对枯草芽孢杆菌发酵生产鸟苷的途径进行分析，确定了3个基本反应模型，计算出最优途径的理论产量和通量分布．三个基本模型的理论摩尔产量分别为0．625、0．75、0．667．通过调整化学调节因子NH4^ 和Mg^2 ，可以使鸟苷产量由14．4g／L提高到19．2g／L。
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| 关 键 词： | 鸟苷 途径分析 代谢通量 枯草芽孢杆菌 |
Patheway Analysis for Production of Guanosine By Bacillus Subtillis |
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| Zhang Bei,Wang Jian,Chen Ning,Zhang Kexu,Hiroshi Asai,Fang Jie.Patheway Analysis for Production of Guanosine By Bacillus Subtillis[J].Acta Scientiarum Naturalium University Nankaiensis,2005,38(1):6-11. |
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| Authors: | Zhang Bei Wang Jian Chen Ning Zhang Kexu Hiroshi Asai Fang Jie |
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| Abstract: | Pathway analysis for production of guanosine by Bacillus subtillis in steady state was conducted in this paper. Three basic modes and flux distribution for optimal pathways were determined, the theoretical molar yield of guanosine in the three mode are 0. 625(e1), 0. 75(e2) and 0. 667(e3)respectively. The yeild of guanosine was improved from 14. 4 g/L to 19. 2 g/L by regulating the chemical regulative factor of NH4 and Mg2 . |
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| Keywords: | guanosine pathway analysis metabolic flux Bacillus subtillis |
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