High K+-induced release of somatostatin from the cortical preparation of rat brain |
| |
Authors: | S L Lee V Havlicek A E Panerai H G Friesen |
| |
Institution: | (1) Department of Physiology, University of Manitoba, 770 Bannatyne Avenue, R3E OW3 Winnipeg, Manitoba, Canada |
| |
Abstract: | Summary Release of endogenous somatostatin (SRIF) from the rat cerebral cortical slices incubated in Krebs-bicarbonate buffer was increased from the basal rate of 3.4±0.6% of the total SRIF content in 15 min at K+]o=5.6 mM, to 13.1±1.6% upon raising the K+]o to 56.6 mM. The high-K+ evoked SRIF release was absent when Ca++ in the medium was replaced by Mn++. The isolated synaptosomes from rat cerebral cortex contain 13.2±3.1 ng SRIF/mg protein compared to 0.33±0.01 ng/mg protein in the cortical tissue as a whole, suggesting that nerve terminals are the main source of the peptide released upon membrane depolarization.The study was supported by a grant from the Medical Research Council of Canada. Results of this work have been published in part as abstracts: Can. Physiol.9, 45 (1978), and Fedn Proc.37, 665 (1978).The authors are greatly indebted to Dr M. Gotz and the Ayerst Research Laboratories for the most generous supply of the synthetic somatostatin. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|