枯草芽孢杆菌表达系统及其启动子的研究进展

枯草芽孢杆菌(Bacillus subtilis)是工业应用中一种重要的模式菌株,具有非致病性、遗传背景清晰、分泌蛋白能力强、容易分离培养等特性,是异源蛋白表达和分泌的理想宿主。高效可控的启动子是实现外源蛋白高效表达的关键因素之一。根据诱导机制,启动子可分为组成型启动子、诱导型启动子、自诱导启动子和时期特异性启动子。本文介绍了枯草芽孢杆菌表达系统、芽孢杆菌σ因子类型及枯草芽孢杆菌启动子的结构,比较了常用启动子的优缺点,总结了新启动子克隆和改造及生物信息学预测启动子的方法,并对枯草芽孢杆菌启动子未来的研究方向进行展望,为进一步研究启动子的结构和功能及工业生产外源蛋白奠定基础。

Research Progress of Bacillus subtilis Expression System and Its Promoter Regulatory Elements

Bacillus subtilis is an important model strain in industrial application.It has the characteristics of non-pathogenicity, clear genetic background, strong protein secretion ability, easy isolation and culture.It is an ideal host for heterologous protein expression and secretion.Efficient and controllable promoters are one of the key factors for the efficient expression of heterologous proteins.According to the induction mechanism, promoters can be divided into constitutive promoters, inducible promoters, auto-inducible promoters and stage specific promoters.This article briefly introduced the Bacillus subtilis expression system, Bacillus sigma factor type and Bacillus subtilis promoter structure, compared the advantages and disadvantages of commonly used promoters, summarized the methods for the cloning and transformation and bioinformatics prediction of new promoters, prospected the future research direction of the Bacillus subtilis promoter which laid the foundation for further research on the structure and function of promoters and industrial production of foreign aid proteins.