以线虫为例研究复方左卡尼汀的药理及毒理作用

以秀丽新杆线虫作为模式生物,研究w(左卡尼汀)=0.1%,w(曲美他嗪)=0.000 5%及两种单药的复方药物复方左卡尼汀对连续三代线虫寿命与生殖能力的影响,并对线虫总脂肪进行油红O染色和气相色谱分析.结果表明:w(左卡尼汀)=0.1%,w(曲美他嗪)=0.000 5%及复方左卡尼汀对连续三代线虫的寿命均影响较小;左卡尼汀和曲美他嗪对亲代、F1代和F2代线虫的生殖能力均产生负面影响;复方左卡尼汀对线虫的生殖能力具有保护作用;限制饮食可减少线虫的产卵量,并与线虫的脂肪累积有关;左卡尼汀和曲美他嗪可减少线虫体内的脂肪累积与总脂肪酸的体积分数,复方左卡尼汀对线虫体内脂肪累积和总脂肪酸的体积分数影响较小.

Pharmacologic and Toxicology Action of Compound L-Carnitine in Caenorhabditis elegans

Taking Caenorhabditis elegans worm as a model organism, we examined the effects of L-carnitine, trimetazidine, compound L-carnitine on the lifespan and reproductivity of the worms. Our results show that w(L-carnitine)=01%, w(trimetazidine)=0000 5% and compound L-carnitine did not change the lifespan of C. elegans. On the other hand, w(L-carnitine)=01%, w(trimetazidine)=0000 5%  significantly reduced the brood size in parental generation, F1 and F2. However, compound L carnitine has no effect on the reproductivity of the worms. Worms undergoing DR utilize fat, and diminish reproductive output. That is to say, the worms with restricteddietary intake have reduced broods. Our results indicate that w(L-carnitine)=01% and  w(trimetazidine)=0000 5% reduce both fat accumulation and overall fatty acid. However, compound L-carnitine has no effect on the overall fat and fat acid.