| 幼叶组织培养在小麦远缘杂交育种中的意义 |
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| 引用本文： | 李素芳,吴兰佩.幼叶组织培养在小麦远缘杂交育种中的意义[J].首都师范大学学报(自然科学版),1989,10(2):35-40. |
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| 作者姓名： | 李素芳 吴兰佩 |
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| 作者单位： | 北京师院
(李素芳)，北京农业大学
(吴兰佩)，中国科技大学(成浩) |
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| 摘 要： | 本文研究了自交不结实或结实率低的小麦×小偃麦、小麦×黑麦和小黑麦×黑麦杂种F_1的幼穗、幼叶、幼茎的组织培养。从幼穗和幼叶的愈伤组织中分化出了再生苗。在实验过程中,比较了不同培养基和不同外植体的诱导和分化的差异。以MS 2mg/L 2,4-D 2.5mg/L Tordon的出愈率最高,以MS 2mg/L 6-BA 0.2mg/L IAA对愈伤组织的分化最为有利。从幼穗再生苗中还得到一株叶片呈白绿条纹的突变。
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| 关 键 词： | 幼叶组织培养 远缘杂交 无性繁殖 染色体加倍 |
The Tissue Culture of Young Leaves for Wheat Distant Cross Breeding |
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| Li Sufang.The Tissue Culture of Young Leaves for Wheat Distant Cross Breeding[J].Journal of Capital Normal University(Natural Science Edition),1989,10(2):35-40. |
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| Authors: | Li Sufang |
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| Institution: | Li Sufang (Department of Biology) Wu Lanpei (Beijing Agriculture University) Cheng Hao (University of Science and Technology of China) |
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| Abstract: | The young inflorescences, leaves and stems of hybrid between wheat and rye, agpyron or between triticale and rye which are sterile or low seed-set,are cultured. Shoots are regenerated from calli initiated from young inflorescence and leaf explants. In the course of experiment, the rates of callus induction among the different mediums and those of shoot differentiation among various explants are compared. There is a mutation of regenerated plants which have white stripes on its leaves. |
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| Keywords: | young leaves culture distant cross vitro propagation chromosome doubling |
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