| 高效降解秸秆纤维素菌株的筛选鉴定及产酶条件优化 |
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| 引用本文： | 王晓林,张西玉,白方文,王魁荣,陈晓敏,白林含. 高效降解秸秆纤维素菌株的筛选鉴定及产酶条件优化[J]. 四川师范大学学报(自然科学版), 2011, 34(1): 105-109. DOI: 10.3969/j.issn.1001-8395.2011.01.022 |
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| 作者姓名： | 王晓林 张西玉 白方文 王魁荣 陈晓敏 白林含 |
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| 作者单位： | 1. 四川大学生命科学学院,四川,成都,610065
2. 乐山师范学院化学与生命科学学院,四川,乐山,614000 |
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| 基金项目： | 科技部支撑计划“秸秆植生带制造技术与产业化示范”(2007BAD39B04)资助项目 |
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| 摘 要： | 从堆沤秸秆土壤中筛选到一株高产纤维素酶菌株YA-14,经菌株形态特性分析和18SrRNA基因序列分析,确定其为烟曲霉(Aspergillus Fumigatus).通过单因素及正交试验,研究了多种因素对菌株产酶效果的影响,确定最佳产酶条件为碳氮比2:1,初始pH值为5,Mg2+ 浓度为0.4 mg/mL,接种量为1.0...
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| 关 键 词： | 秸秆 烟曲霉 纤维素降解 产酶条件优化 固体发酵 |
Screening, Identification and Optimization of Fermentation Conditions for a High Efficiency Straw-degradation Strain |
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| WANG Xiao-lin,ZHANG Xi-yu,BAI Fang-wen,WANG Kui-rong,CHEN Xiao-min,BAI Lin-han. Screening, Identification and Optimization of Fermentation Conditions for a High Efficiency Straw-degradation Strain[J]. Journal of Sichuan Normal University(Natural Science), 2011, 34(1): 105-109. DOI: 10.3969/j.issn.1001-8395.2011.01.022 |
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| Authors: | WANG Xiao-lin ZHANG Xi-yu BAI Fang-wen WANG Kui-rong CHEN Xiao-min BAI Lin-han |
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| Affiliation: | WANG Xiao-lin1,ZHANG Xi-yu2,BAI Fang-wen1,WANG Kui-rong1,CHEN Xiao-min1,BAI Lin-han1(1.College of Life Sciences,Sichuan University,Chengdu 610065,Sichuan,2.College of Chemistry and Life Sciences,Leshan Teacher's College,Leshan 614000,Sichuan) |
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| Abstract: | A high-efficient cellulose-degradation stain was screened from the soil of rotten straw.The selected strain(YA-14) was identified as Aspergillus fumigatus according to the 18S rRNA gene sequencing and phylogenetic analysis.Single factor experiment and orthogonal test were designed to optimize the fermentation conditions of cellulase production.The results showed that the optimum conditions of enzyme production were 2∶1 of C/N,pH=5.0 at the beginning of the culture,0.4 mg/mL of Mg2+ concentration,and 1.0% of inoculation.In the optimizing conditions,after 72 h cultivation,the cellulase activity of the strain was 0.486 IU/mL,being 2.68-times as against 0.181 IU/mL before the optimization.The results of solid-state fermentation showed that this strain had a high efficiency of straw cellulose degradation. |
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| Keywords: | straw Aspergillus fumigatus cellulose-degradation optimization of fermentation conditions solid-state fermentation |
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