| 金属离子和脲对背角无齿蚌碱性磷酸酶的影响 |
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| 引用本文： | 张洪渊 刘克武. 金属离子和脲对背角无齿蚌碱性磷酸酶的影响[J]. 四川大学学报(自然科学版), 1996, 33(1): 100-105 |
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| 作者姓名： | 张洪渊 刘克武 |
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| 摘 要： | 研究了多种金属离子对背角无齿蚌碱性磷酸酶（ＡＫＰ）活性的影响，结果表明：Ｎａ＾＋，Ｋ＾＋和Ｌｉ＾＋等一价金属离子没有影响：Ｍｇ＾２＋，Ｃａ＾２＋，Ｂａ＾２＋，Ｎｉ＾２＋，Ｍｎ＾２＋和Ｃｏ＾２＋有激活作用；而Ｃｕ＾２＋，Ｚｎ＾２＋，Ｈｇ＾２＋，Ｃｄ＾２＋及Ａｇ＾＋有抑制作用，脲对ＡＫＰ的变性失活作用，按脲浓度可为低于２ｍｏｌ／Ｌ和高于２ｍｏｌ／Ｌ两种类型，低浓度脲对背角无齿蚌ＡＫＰ活性抑制的动力学表
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| 关 键 词： | 金属离子 脲变性失活 碱性磷酸酶 背角无齿蚌 |
EFFECTS OF METAL IONS AND UREA ON THE ALKALINE PHOSPHATASE FROM WOODIANA HEUDE |
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| Zhang Hongyuan,Liu Kewu ,Gon Youbin Hu Mei,Luo Shengqing. EFFECTS OF METAL IONS AND UREA ON THE ALKALINE PHOSPHATASE FROM WOODIANA HEUDE[J]. Journal of Sichuan University (Natural Science Edition), 1996, 33(1): 100-105 |
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| Authors: | Zhang Hongyuan Liu Kewu Gon Youbin Hu Mei Luo Shengqing |
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| Affiliation: | Depertment of Biology |
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| Abstract: | The survey of effects of metal ions on the alkaline phosphatase(AKP)activity from Anodonta woodiana Heuds shows that Na ,K and Li have not any effect on the enzyme activity, Mg2 ,Ca2 ,Ba2 ,Ni2 , Mn2 and Co2 aotivate the enzyme,Cu2 ,Zn2 ,Hg2 ,Cd2 and Ag inhibitthe enzyme.The AKP is inactivated by urea,and the inactivation can be divided into two kinds according to the concentration of the urea:lower than 2 mol/L and higher than 2mol/L. At lower concentration,effect of urea on the enzyme displayed a mixed tyme of inhibition. |
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| Keywords: | metal ions urea inactivation alkaline phosphatase Anodonta Woodiana Heude |
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