| rhGM-CSF/IL-3融合蛋白中残余菌体DNA的检测 |
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| 引用本文： | 邱雪莲,陈敏.rhGM-CSF/IL-3融合蛋白中残余菌体DNA的检测[J].汕头大学学报(自然科学版),1999,14(2):77-80. |
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| 作者姓名： | 邱雪莲 陈敏 |
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| 作者单位： | 华茵生物工程公司!汕头．５１５０４１ |
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| 摘 要： | 本文利用地高辛标记及检测试剂盒，标记ｐｏｐ２１３６（ＰＭＴＧ３）工程菌染色体ＤＮＡ作为探针，检测基因工程药品重组人粒细胞－巨噬细胞集落刺激因子／白细胞介素－３（ｒｈＧＭ－ＣＳＦ／ＩＬ－３）融合蛋白中残余的菌体ＤＮＡ．结果符合质量标准每剂＜１００ｐｇ．
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| 关 键 词： | 地高辛 标记 探针 杂交 G3 |
Detection of residual DNA from E. coli in recombinant human granulocytic-marcophagic colony stimulating factor/interleukin-3 fusional protein |
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| Qiu Xuelian, Chen Min, Sheng Xiaofang.Detection of residual DNA from E. coli in recombinant human granulocytic-marcophagic colony stimulating factor/interleukin-3 fusional protein[J].Journal of Shantou University(Natural Science Edition),1999,14(2):77-80. |
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| Authors: | Qiu Xuelian Chen Min Sheng Xiaofang |
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| Abstract: | In this paper, pop2136 (PMTG3) chromosomal DNA labeled by the markerreagent digoxin is used as a probe to detect the genetic engineering pharmaceutic calledrecombinant human granulocytic-marcophagic colony stimulating factor/ interleukin-3(rhGM-CSF/Il-3) fusional protein. The findings obtained are up to the quality control.standard of lower than 100pg. |
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| Keywords: | digoxin label probe hybridization recombinant human granulocyticmarcophagic colony stimulating factor/ interleukin-3 fusional protein (G3) |
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