祁连龙胆愈伤组织和再生植株的生长及其两种药效成分分析

通过不同激素组合的培养基筛选出祁连龙胆（Gentiana Przewalskii Maxim.）的快速生长细胞系并获得再生植株. 采用反相高效液相色谱法测定愈伤组织及其再生植株中獐牙菜苦甙（Swertiamarin）、龙胆苦甙(Gentiopicroside)的含量. 结果表明：(1）祁连龙胆愈伤组织在不同培养基上诱导率和生长速度不同，在MS+2?mg/L 2,4 D +1?mg/L 6 BA +6?mg/L GA的培养基上诱导率最高，达78.0％. 在MS+3?mg/L 2,4 D +0.5?mg/L KT的培养基上生长最快，增殖倍数达到7.18； (2）祁连龙胆愈伤组织及其再生植株有效成分及含量与原植株相比均有差别，其中，愈伤组织含有0.265%的龙胆苦甙，低于原植株(4.225%)，再生植株含有0.279%的獐牙菜苦甙和1.579%的龙胆苦甙，前者高于原植株（0.260％），但后者略有下降. 按生长速度推算，愈伤组织及其再生植株积累的药效成分含量明显高于原植株，值得进一步开发利用.

The growth of calli and regenerated plantlets of Gentiana Przewalskii Maxim. and the constituents analysis of its two effective components

Vigorous grown embryonic calli and regenerated plants from Gentiana Przewalskii Maxim., a Chinese medicinal plant, are obtained by various media with different hormone combinations. The contents of swertiamarin and gentiopicroside of embryogenic calli, the regenerated plantlets and the wild plants in G. Przewalskii were determined by reverse phase high performance liquid chromatography (RP HPLC). The results show the induction and the growth rates of calli cultured on various media are different. In addition, the highest frequency (78.0%) of calli induced is observed on MS medium with 2?mg/L 2,　4 D, 1?mg/L 6 BA and 6?mg/L GA, while the fastest growth of embryonic calli is occurred on MS medium with 3mg/L 2,4 D and 0.5?mg/L KT, which has increased 7.18 fold. Furthermore, the effective components in embryonic calli and the regenerated plants are different. The embryonic calli contains 0.265% of gentiopicroside, which is lower than that of the wild plant. The regenerated plants have 0.279% of swertiamarin and 1.579% of gentiopicroside, of which the former is higher and the latter is lower than that of the wild plant. Base on the speed of growth, the contents of the effective components accumulated from the calli and the regenerated plants are much higher than that from the wild plants.