地黄花药培养再生植株

把花粉处于单核或双核初期的花药接种在MS培养基上,培养基附加2,4—D0.5mg/l、NAA0.5—1mg/1、6—BA2mg/l、水解乳蛋白500mg/l及蔗糖3％诱导愈伤组织的形成。MS培养基附加6—BA2mg/l、NAA0.5mg/J及蔗糖3％诱导愈伤组织的分化。经过一个阶段的培养再生出地黄小苗。是否为单倍体植株有待组织细胞学工作鉴定。再生植株进行继代培养后,在茎上长出若干不定根,可能为药用植物的品种改良和药物生产提供了一条途径。

ANTHER CULTURE OF REHMANNIT GLUTINOSA AND REGENERATION OF PLANTLETS

An theres of rehmannia glutinosawhose pollen grains were at the state of monokaryon or early stage of dikaryon were cultured on MS media supplemented with2, 4—D0.5mgL NAAO.5—1mg/1、6—BA0.5mg/1、LH500mg/1 and sucrose 3% for the induction of callu~s formation; or supplimented with 6—BA 2mg/1、NAA0.5mg/1 and sucrose 3% for the ninduction of callus differentiation into plantlets, Nevertherless, Histocytolo gical work eeds to be done for determining whether the Plantlets are haploid or diploid plants.—But from the tender stems of the plantlets which were cultured generation by generation grew a number of adventitious roots, which might inaugurate a new way to improve sP—ecies of medicinal plants and to produce medicines