| 钙调素拮抗剂TFP对BGC-823细胞增殖的影响及其分子机制的探讨 |
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| 引用本文： | 张伟,彭丽霞,柳惠图,高萍. 钙调素拮抗剂TFP对BGC-823细胞增殖的影响及其分子机制的探讨[J]. 北京师范大学学报(自然科学版), 2003, 39(1): 115-119 |
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| 作者姓名： | 张伟 彭丽霞 柳惠图 高萍 |
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| 作者单位： | 北京师范大学细胞增殖与调控生物学教育部重点实验室,100875,北京 |
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| 基金项目： | 国家重点基础研究发展计划(973计划);G1999053901; |
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| 摘 要： | 为了进一步探讨钙调素拮抗剂TFP对人胃癌细胞增殖的影响及其分子机制，利用MTT法及流式细胞光度术检测了TFP对细胞增殖的影响，进一步利用western blot的方法对细胞中p16^INK4a和cyclinD的表达水平进行了检测。结果表明：TFP处理可以明显提高BGC－823细胞中p16^INK4a的表达水平，而cyclinD1的水平则明显下降，提示TFP可能通过影响p16^INK4a的表达水平抑制细胞的增殖。
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| 关 键 词： | 钙调素 三氟拉嗪 TFP p16^INK4a 细胞周期 |
| 修稿时间： | 2002-07-20 |
EFFECT OF TFP, A INHIBITOR OF CALMODULIN, ON CELL PROLIFERATION IN BGC-823 CELLS AND IT''S MOLECULAR MECHANISM |
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| Zhang Wei Peng Lixia Liu Huitu Gao Ping. EFFECT OF TFP, A INHIBITOR OF CALMODULIN, ON CELL PROLIFERATION IN BGC-823 CELLS AND IT''S MOLECULAR MECHANISM[J]. Journal of Beijing Normal University(Natural Science), 2003, 39(1): 115-119 |
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| Authors: | Zhang Wei Peng Lixia Liu Huitu Gao Ping |
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| Abstract: | To investigate the inhibition mechanism of calmodulin inhibi to r TFP in the proliferation of BGC-823 cells, the effect of TFP on the prolifera tion of BGC-823 and the level of p16INK4a and cyclinD are assayed respect ively by MTT, FCM and western blot. These results show that TFP inhibits the pro liferation of BGC-823 cells. The level of p16INK4a increases great ly. On the contrary, the level of cyclin D1 decreases obviously. This may be on e of the melecular mechanisms which is involved in the regulation of proliferati on in BGC-823 cells by TFP. |
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| Keywords: | calmodulin trifluoperazine (TFP) p16INK4a cell cycle |
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