| 籼稻“成恢448”组培体系的建立 |
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| 引用本文： | 孙青芝,吕冰洁,马伯军.籼稻“成恢448”组培体系的建立[J].浙江师范大学学报(自然科学版),2014(3):323-328. |
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| 作者姓名： | 孙青芝 吕冰洁 马伯军 |
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| 作者单位： | 浙江师范大学化学与生命科学学院,浙江金华321004 |
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| 基金项目： | 国家自然科学基金资助项目（31371593;31101130） |
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| 摘 要： | 对籼稻"成恢448"愈伤组织的诱导和分化过程进行了优化条件的探索.通过正交实验设计了不同的激素配比,结果显示:最佳的愈伤组织诱导条件为MS培养基加入1.0 mg/L 2,4-二氯苯氧乙酸(2,4-D),4.0 mg/L萘乙酸(NAA)和2.0 mg/L激动素(KT);最佳的分化条件为MS培养基加入1.0 mg/L NAA,2.0 mg/L 6-苄氨基腺嘌呤(6-BA)和0.5 mg/L KT.
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| 关 键 词： | 籼稻 成恢 组织培养 激素配比 |
Establishment of tissue-culture system for a rice indica cultivar Cheng-hui 448 |
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| SUN Qingzhi,LU Bingjie,MA Bojun.Establishment of tissue-culture system for a rice indica cultivar Cheng-hui 448[J].Journal of Zhejiang Normal University Natural Sciences,2014(3):323-328. |
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| Authors: | SUN Qingzhi LU Bingjie MA Bojun |
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| Institution: | ( College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua Zhejiang 321004, China) |
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| Abstract: | Rice indica cultivar Cheng-hui 448, an excellent restorer line in hybrid rice , had resistant to the blast while sensitive to the bacterial blight .Recently, with transgenic technology , a number of resistant genes were applied in rice breeding .However , the tissue-culture systems of indica were still imperfect and signifi-cantly different in varieties .Here, optimization conditions of callus induction and differentiation for the indica variety Cheng-hui 448 was experimented by orthogonal test .According to the results , MS media with 1 .0 mg/L 2,4-D, 4.0 mg/L NAA and 2.0 mg/L KT, MS media with 1.0 mg/L NAA, 2.0 mg/L 6-BA and 0.5 mg/L KT, were the optimization conditions for callus induction and differentiation of Cheng-hui 448, respec-tively . |
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| Keywords: | indica tissue-culture hormone ratio |
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